Studies On The Cold-Resistance Ability And Cloning And Expression Of SAD Gene Of Jatropha Curcas

Jatropha curcas L, belonging to Euphorbiaceae and thriving in many areas of the tropics and sub-tropics around the world, is a multipurpose plant with many attributes and considerable potentials. However, its utilization is limited by the only tropics and sub-tropics distribution. Stearoyl-acyl carrier protein (ACP) desaturase (SAD, EC.1.14.99.6) catalyzing desaturation of stearoyl-ACP to form oleoyl-ACP is a key enzyme of fatty acids synthetic metabolism in higher plants and plays a key role in determining the ratio of saturated to unsaturated fatty acids in plants. It was reported that this ratio in membrane lipids was closely related to many functions, especially the stress resistance of plants. The available information indicates that the chilling resistance of plants rises with the increase in the contents of the unsaturated fatty acids in membrane lipids. Here the cold-resistance ability of J. curcas was first studied, which may lay the basis for selecting cold-resistance varieties of J. curcas. Then, the gene of J. curcas SAD (JSAD) is cloned and...