Generation Of Human Monoclonal Antibodies Against HFRS Virus By Phage Display Technology And Construction Of Eukaryotic Expression Vectors Of The Antibody Genes

Hemorrhagic fever with renal syndrome is a kind of severe infectious disease caused by virus, and it still can't be cured effectively. Mouse mAbs have been applied in basic medical research and clinic diagnosis extensively since 1980s, but its application in humanbody is limited because mouse mAbs can induce a human anti-mouse antibody response when being used in vivo. Since its invention at the end of 1980s, antibody phage display has revolutionized the generation of monoclonal antibodies and their engineering. It is now possible to create human antibodies binding to any chosen target antigen without the use of laboratory animals or hybridomas, in a system that completely by-passes the immune system.Three parts of work were included in our research. The first, a phage display library of human antibody was constructed as follow. The total cell RNA extracted from peripheral lymphocytes of an individual who was convalescent from HFRS was transcribed reversely into cDNA. The genes of human immunoglobulin heavy chain Fd and light chain were amplified by polymerase...