A Study On Three Dimensional Protein Structure Based On Structural Spectrum

One of the aims of Human Genome Project is to elucidate the structures, functions and internal interactions of about a hundred thousand proteins of human being as well as their relations with various diseases. The three dimensional structure of protein is close related with it's function, thus the study of protein structure is the core issue of Proteomics. However, comparing three-dimensional structures between proteins is a task of computer graphics. The dissertation focuses on key techniques of such comparison, which is done in both global and local sense and proposes efficient methods in multi-level comparison of protein's spacial structures. The research works are as follows:1. With an integration of data mining, computer graphics and digital image processing, the dissertation first examines the data normalization of protein's three dimensional structures, then feature extraction and the multi-level comparison of three dimensional structures, and finally proposes a mechanism of carrying out the protein's spacial comparison in both global and local sense.2. Visualization of protein's three-dimensional structure.3. By means of analyzing the protein's principal component with second-order moments, establish the three-dimensional unified coordinates of proteins. Under the coordinates, implement wavelet transform to build the multi-scale spectrum of the spatial structure that represents protein'sα-carbon distance and its coordinate sequence respectively. Thus, the protein's multi-level features are extracted.4. Utilize the grey incidence theory to compare the protein's spacial structure and then analyze the protein's structural spectrum, which comprehensively describe the similarities of the protein's spacial structures in multi-scale sense.5. Implement fractal analysis to the similarities of the protein's spacial structure spectrum, which shows another way of multi-level comparison between proteins.This research is funded partly by the National Natural Science Foundation of China and fully by dedicated research plan of Shaanxi education bureau.