Hdrogen Production From A Two-step Process Of Acidogenic Bacteria Fermentation And Photosynthetic Aacteria Fermentation

In recent years, many researchers were focus on biohydrogen production by fermentation the waste water and treated waste. It is crucial to acquire high hydrogen-producing microorganisms for biological hydrogen production on biohydrogen production. Therefore, we utilized different methods to acquire high hydrogen-producing acidogenic microflora and photosynthetic microflora from marine environment.In this study, high hydrogen-producing acidogenic bacteria microflora was acquired through different pretreatment methods, for example acid pretreatment, alkali pretreatment, heat-shocked pretreatment and nitrate pretreatment. Among all these pretreatment methods, the microflora from heat-shocked pretreatment obtained the highest hydrogen yield and maximal hydrogen production rate. Initial pH and sucrose concentration affected on the hydrogen production of acquired hydrogen-producing microflora. The heat-shocked pretreated microflora achieved highest hydrogen production at the initial pH 8.0, which the pH was close to natural sea water pH value. It means that the microflora could be an candicate engineering microflora for hydrogen production by treating marine waste water.On the other hand, two groups of marine photosynthetic hydrogen-producing microflora were acquired, which could respectively utilize acetate and butyrate as carbon source to produce hydrogen. Temperature, light intensity, initial pH and initial carbon source concentration had great influence on the growth and hydrogen production of two bacterial group. Higher hydrogen production and substrate utilization efficiency were obtained in sequential test of hydrogen production by acidogenic microflora and photosynthetic microflora.In addition, and easy and fast method was found to isolate anaerobic hydrogen-producing bacteria. 61 strains of anaerobic acidogenic bacteria and 34 strains of photosynthetic bacteria were isolated from the above-mentioned acidogenic and photosynthetic microflora. Among these bacteria strains, 12 strains of anaerobic acidogenic bacteria and 7 strains of photosynthetic bacteria had higher hydrogen production.In conclusion, many works were done for biohydrogen production by marine bacteria in our study. Utilizing marine aquaculture wastes and waste water as hydrogen production substrate to hydrogen production could combine biohydrogen production with wastes treatment. The prerequisite for biohydrogen production by treat mariculture waste and waste water was solved in this study.