Innovation Of Gene Mapping Methods And Investigation On The Hardware Of DNA Automata

Genotyping, statistical analysis and gene function research are the three key parts of modern genetics. This three parts linking in turn becomes an important method for discovering the mechanism of genetic dieases. In this thesis, the research methods in these three parts were analyzed and improvement was presented to solve the existing problems. At first, a novel AuNP assisted allele specific PCR was presented to enhance the genotyping ability of ASPCR. Because AuNP is cheap and the original protocol is changed little, this method is hopeful to become a new cost-efficient genotyping method. At second, the sib-pair linkage analysis method is improved based on the Haseman-Elston model to be applied to ordinal trait loci mapping. The data simulation results showed that the new linkage analysis method is optimal for small sample with strong linkage. At last, the ligase dependency of type II restriction enzymes when performing DNA automaton computing was investigated based on the experiment result. Moreover, DNA automaton could also be used to regulate gene expression in vitro logically. This provided a novel method for gene function analysis. From the result, we can see that FokI is the most suitable DNA automaton hardware, but other enzymes have their advantages. This investigation supported the development to enzyme-free DNA automaton.