Comparative Analysis Of Rice Full-Length CDNAs, Construction Indica CDNA Database, And Genome-wide Investigation The Trihelix DNA-Binding Proteins Plants Animals

Rice is one of the principal foods of the world. Asian cultivated rice (Oryza sativa) that composes of two major subspecies, japonica and indica, occupies the largest area of cultivated rice production in the world. Japonica subspecies distributes primarily in temperate zone of south China, while indica subspecies is primarily distributed in east of India, the tropic and subtropics. Nowadays, indica subspecies occupies larger area of rice production than japonica.There are plenty of rice japonica and indica genome sequences and japonica expressed sequences (especially rich in full-length cDNA sequences) available in public database. However, there are few indica and wild rice expressed sequences, especially short of full-length cDNAs. Hence, it is essential to collect, sequencing and analysis of massive indica and wild rice expressed sequences. Not only will it provide important gene resources to researchers, but also it will be valuable for comparative analysis between indica and japonica, and between cultivated rice and wild rice as well.In addition, phylogeny construction among some gene families is also an effective means in functional genomics analysis. It is helpful for identification of gene families and evaluation its putative function.There are two major parts in this dissertation. The first part includes analysis of indica Guangluai-4 and wild rice W1943 massive full-length cDNAs and the sequential database construction; the second part includes genome-wide investigation of the trihelix DNA-binding proteins.