| The processes of cellulases induction from Trichoderma reesei RUT C30 acted on soluble carbon sources or cellulose materials were studied. From the results, it can be said that cellooligosaccharides had nearly no induction ability. The highest cellulase activity of 11.2 FPIU/ml was accquired on acid–hydrolyzed flour of 28.85 g/L. Acid–hydrolyzed flour was mainly composed of isomaltose, gentiobiose and cellobiose by HPAEC-PAD analysis. Saccharides consumption of acid–hydrolyzed flour during the cellulase fermentation was also analyzed. It was found that 46.1 % of cellulase was synthesized after gentiobiose, cellobiose maltose and sophorose were exhausted, which convinced that isomaltose took part in cellulase induction. Hydrolysis processes of acid–hydrolyzed flour and isomaltose by cellulase,β-glucosidases, mycelium and intercellular enzyme were also performed, the results showed gentiobiose and cellobiose can not be used as cellulase inducers because of their quickly consumption, while isomaltose can hardly be degraded by cellulase andβ-glucosidases, and can realize its induction ability by partly transforming to other disaccharides, like sophorse.Cellulose with different crystallinity and structure was obtained by processing with sulfuric acid and sodium hydroxide. When cellulase produced on 10 g/L cellulose, the results of cellulase activity indicated that the more cellulase productivity can be achieved on the higher crystallinity cellulose materials. The lowest and the highest productivity of cellulase of 2.8 and 6.3 FPIU/ml were obtained on the crystallinity index cellulose of 28.1 % and 63.1 % individually. However, the production ofβ-glucosidases were more depended on cellulose structure, which produced more than 1.4 IU/ml on the carbon sources with cellulose I structure and less than 0.6 IU/ml on that with cellulose II structure.The components of culture mediums and intercellular materials of T. reesei acting on pulp, Sloka Floc, MCC and acid-hydrolyzed pulp were studied in details by HPAEC-PAD. It was found that there were glucose, xylose, cellobiose, sophorose, isomaltose and maltose in the culture mediums in trace amounts, and isomaltose was the predominant disaccharides. The hydrolyzation of those four cellulose materials by cellulase and mycelium were analyzed. It's found that glucose, xylose and cellobiose were the main products when them hydrolyzed by cellulase, the transglysation products of sophorose, isomaltose and maltose were also be detected. However, the transglysation products became dominants when they hydrolyzed by mycelium. After 48 h of hydrolyzation, the transglysation products were taken part in 80.0,92.3,84.6 and 71.8 %, and among them there were 33.3,32.2,54.7 and 56.9 % of isomaltose. The results showed isomaltose was the mainly transglycosylation product of cellulose degradation and took part in cellulase induction by solid carbon sources. It was also showed those carbion sources had better cellulase productivity can produce more isomaltose.The protein compositions of the secretome produced on kinds of carbon sources were explored using two-dimensional electrophoresie. With cellulose as the carbon source, enzymes can be detected from 150 to 170 protein spots, in addition to cellulase components of CBH I, CBH II, EG I and EG II, hemicellulase components of Cel 74A, MAN, Axe1, BXL I, XYN I, XYN IV, Agl I, ABF and non-hydrolyzed protein SWO, CIP I, CIP II also existence. However, when alkali treatment pulp was used as carbon source, the enzymes did not contain Axe1, EG IV and EG III, and EG III with pI 7.5 also had almost disappeared. There were only 110 spots be detected on the 2D map of enzyme induced by acid–hydrolyzed flour, which in the absence of EG III with pI 5.0, Agl I and CIP II and other proteins, but EG IV, never found in solid carbon sources, was showed.
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