Study On The Application Of Water - Soluble Rare Earth To Nano - Material For Small Animal Live Imaging

The development of new biocompatible rare earth upconversion luminescence nanomaterials (UCNPs) for upconversion luminescence bio-imaging guidance surgery treatment has become the field of biological imaging problems to be solved. For this scientific problem, we have developed a new biocompatible upconversion rare earth nanomaterials for different research includes the following sections:(1) By SPECT imaging polymer coated radioactive rare earth nanomaterials biodistribution and metabolism. We use different polymers (PEI, PAA, PVP and PEG) as a co-ligand to directly synthesized radioactive and ultra-small (<10nm) rare earth upconversion luminescence nanomaterials NaYF4:Yb, Er(153Sm). By SPECT imaging the differences polymer coating UCNPs showed difference in the distribution in vivo. Through the blood gamma-counter quantitative analysis, we found that PEG-coated UCNPs (153Sm) compared to other polymer-coated rare earth nanomaterials, has a longer blood retention time, and we found that rare earth upconversion luminescence nanomaterials (UCNPs) could be distributed in the kidneys and metabolism in the urine.(2) Biocompatibility basic carbonate coated core-shell structure of rare earth fluoride nano-materials for biological imaging. We design and development of a rare earth basic carbonate coated core-shell structure of the rare earth fluoride nano-materials. The NaYF4:20%Yb,l%Tm as the core, Gd(OH)CO3as shell and the surface with PEG polymer of PEG-UCNPs@Gd(OH)CO3were obtained. We found that rare earth nano-materials having high stability in the physiological environment. After the tail vein injection, we realized upconversion luminescence imaging of the body’s blood vessels in mice, and the material can be gradually distributed in the body’s muscles and bones.(3) Hyaluronic acid rare earth nanomaterials for lymphatic upconversion luminescence imaging. We designed and synthesized hyaluronic acid-coated NaYF4:20%Yb,l%Tm@NaLuF4@Gd(OH)CO3core-shell structure nanoparticles. We found that this material can be effectively targeted normal mice lymphatic tissue or lymphatic tissue around the tumor in mice (lymphatic vessels and lymph nodes). According to high-resolution and long-time upconversion luminescence imaging, we carried the cancer surgical removal of tumor and metastatic lymphatic vessel to lymph nodes in living mice.