| Traditional Chinese medicine (TCM) is an analytical system with too much complexity. Usually, many chemical components exist in a TCM and most of ingredients are with very low content. As a result, common analytical instruments and detecting approaches can not satisfy the need to qualitatively and quantitatively determine chemical components from a TCM completely. With the advent of two-dimensional hyphenated chromatographic instruments and the rapid development of relative multi-resolution techniques based chemometrics, it is possible that a TCM can be qualitatively and quantitatively analyzed completely due to the advantage of these new approaches both in chroinatographic separation and in spectral identification.However, chromatograpbic peaks with incomplete separation or so-called overlapped peaks containing more than one component commonly present themselves in the analysis of a TCM in practice. The purity of these incomplete peaks should be identified at first and then these peaks are resolved into pure chromatograms and spectra with the aid of multi-resolution techniques based chemometrics. The qualitative and quantitative determination of these chemical components from a TCM can become available by means of pure chromatograms and spectra obtained above.On the other hand, it is impossible to qualitatively and quantitatively determine every component from a TCM accurately on account of its too much complexity. Establishing a chemical fingerprint with many characteristics becomes one of the most effective approaches applied to the quality control and technologic standardization of a TCM in the world so far.In the introduction part of this thesis, a brief review is given to the recent development of TCMs. In the view of the varieties, production, input and output marketing of TCMs, research on natural products gradually becomes one of the hot topics all over the world now. The development of analytical science, which is closely related to the quality control and technologic standardization of TCMs, is also simply introduced in respect that the output of TCMs is being confronted with many problems, especially, the realization of the standardization, modernization and internationalization of TCMs is nota trivial work in our country. The introduction of chemometrics and relative multiresolution methods applied to two-dimensional hyphenated chromatographic instruments certainly provides a novel approach for determining chemical components from TCMs.Most of the water-soluble constitutes of TCMs are efficacious. On the other hand, various parts of a TCM may be different in the water-soluble constitutes. In chapter two, nucleoside components, which are soluble in water, from the caterpillar body and stoma parts of Cordyceps sinesis with special pharmacological activities from Xizang district are qualitatively and quantitatively determined by means of HPLC-DAD in combination with a multi-resolution technique named Heuristic evolving latent projections (HELP). On the other hand, a fat-soluble component ergosterol is also detected by means of the same method. The result obtained in this chapter can direct ones to the reasonable taking of rare and valuable Cordyceps sinesis.Volatile oils from TCMs are important chemical components with some efficacious functions. Constituents in essential oils from Rhizoma atractylodis, Pericarpium citri reticulatae, Cortex rnagnoliae officinalis and Radix glycyrrhi:ae along with their preparation peptic powder are qualitatively and quantitatively analyzed aided by GC-MS combined with some multi-resolution techniques like HELP in chapter three. So are done the chemical components in volatile oils from Rhizoma chuanxiong, Radix angelicae sinensis, Radix paeoniae alba and Radix rehmanniae praeparata with their compound si-wu decoction in chapter four. In the latter chapter, the in-depth researches show the reasonableness of the compatibility of the four single herbs constructing the preparation si-wu decoction.Embedded peaks are usually pr...
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