Study On The High Activity Mechanism, The Adsorption Properties And The Hydrolysis Kinetic Of Xylanase From Sp.E-86 Strain

With the uninterrupted growth of living standard, people recently pay attention not only to thefood's colof, savory flavor and nutritive value but also to health care function. The functionalfOods have been become popular. Since they have some specific physiological functions, such aJspromoting the multiplication of BytdObacleria in intestines, keeping a good healthy for peopIes,the functionaI oligosaccharides have been attached impohant to by the researchers all over theworld. The functional oligosaccharides have become a kind of important functional fOods' basematerials. It's very difficult for xylooligosaccharide's digestion in intestines. The intestinalsurvivors of xylooligosaccharides are too much, and can keep a good multiplication forBpobacteria. The utiliZaion of the xylooligosaccharide is the highest among the otheroligosaccharides. A xylooligosaccharide is composed of 2--7 xyIoses. The link between themolecules is 6-l,4 xylosidate band. lt can be prepared by 0-xylanase reaction with sawdust,corncob, cotton seed hull, rice straw or bagasse. Preparation of xylooligosaccharides fromcorncobs by enZyme system done by Li-Lite who is professor of Sino-Japanese Food ResearchCentef, Chinese Agriculture University has been succeed. It is necessary fOr succeeding andperfecting industrialiZation production of xylooligosaccharides from comcobs by enZyme systemto fully make use of the renewable resources, to protect environment to increase additionalagricultural value and to reduce product cost.This paPer first stUdied the high activity of xylanase from Stroptomyces olivaceovirdis E-86(sp.E-86) on different inducing substrate, the adsorption properties of xylanase from sp.E-86 andshopping hemicellulase on the surfaces of lignocellulosic materials such as cellulose, xylan,lignin, holocellulose and corncob for 90 min at 4C, the hydroIysis of the materials by thedifferent activity amount of xylanase from sp.E-86 at 55C and by shopping hemicellulase at 40t. The embedded research about the mechanisms of producting xylanase from sp.E-86 ondiffereni inducing substr3te, adsorPtion and enZyme reaction had been done. The math models ofthe adsorption isotherms ofxylanase from sp.E-86 and shopping hemicellulase on the surfaces ofthe lignocellulosic materials for 90 min at 4t and the hydrolysis of the materials by thedifferent activity amount of xy1anase from sp.E-86 at 55oC and by shopping hemicellulase at 40OC had been set up. The main detail research contentS are as followingl. The influences of the sp.E-86 strain growing conditions on the different carbon source slantswere observed. The conditions about the xylanase preparation made by the strain growing onthese slants were studied. At the same time, the activities of xylanase from sp.E-86 by differentinducing xyIans were studied too. The experiments'resuIts were as following' 1) The growingconditions of the sp.E-86 strain on the blend of the bran eXtractS and the potato eXtracts slantwere as same as that of on the slant of corncob xylan. The slani could instead of the corncobxyIan sIant on the cuIture of the sp.E-86 strain at a1l. 2) Acid hydrolysis, ion chromatography anddissolution rate of water-insoluble corncob xylan, ethanol-insoluble corncob xylan and birchxylan had been done respectively. The water-insoluble corncob xylan was rnainly linear structure.The ethanol-insoluble corncob xylan had ]inear and branched structures. The birch xylan wasmainly branched. It was greatly influence for the branched sugar and the amount of xylan toinduce xylanase from sp.E-86 strain. 3) The 1.5% water-insolubIe corncob xylan showed a verystrong inductivity to the sp.E-86 strain producing xylanase. The enryme activity, which carbonsource was the water-insoluble comcob xylan, was 3 times higher than that of the l.5% birch- l - --tdi l:';::{,iitk i/ekttoghxylan and 4 times higher than that of the 2.0% ethanol-insoluble corncob xylan. 4) When theamount of c...