Researches On Biocompatible Core-Shell Nanoparticles And Their Applications In Biology And Medicine

According to the advanced directions of the bionanotechnology, four parts of investigations has been performed in this thesis. In part one, the synthesis methods and mechanism of core-shell nanoparticles have been investigated. Great breakthrough in the synthesis theory and techniques of core-shell nanoparticles has been obtained. The results have testified that the charges of the shell materials and core materials will dominate whether the stable core-shell structure forms or not, by employing the silica shell formed through the hydrolysis of silane agents and the core formed by microemulsion. Under the instruction of the synthesis mechanism theory of core-shell nanoparticles, a series of making-technique of core-shell nanoparticles have been developed. A series of core-shell nanoparticles with own intellectual property have been prepared, which provides theory for the magnified synthesis of core-shell nanoparticles. In part two, the biocompatibility of the core-shell nanoparticles, including silica nanoparticles, fluorescent silica nanoparticles and silica coated magnetic nanoparticles, have'been investigated from the angle of in vivo and ex vivo. In part three, the physical and chemical properties of core-shell nanoparticles have been characterized by using transmission electronic microscopy, atomic force microscopy, zetasizer and alternating grade magnetometer. In part four, new biological analytical methods based on core-shell bio-nanoparticles have been established and the analysis of biological medicine in suit, in vivo, in real time have been developed under the combination of nanotechnology and biotechnology. (1) Biological and chemical fluorescent sensing at nano-scale have been developed. One is the novel fluorescent label method based on core-shell fluorescent nanoparticles. We have recognized the SmlgG+B lymphocyte and detected the Systematical Erythema Lupus by using of this novel label. The other is a developed nano-pH sensor based on the fluorescent nanoparticles. The pH changes in single murine macrophages have been measured by using the nano-sensor. (2) Super paramagnetic nano-captures based on silica coated magnetic nanoparticles have been developed. These captures were successfully used to separate substances like glucan and DNA. (3) A novel DNA enriching technique based on amino-modified silica nanoparticles have been established. A novel non-viral genecarrier with protective ability from DNasel and a rapid DNA extraction and purification method have been developed based on the novel DNA enrichment technique.