Decolorization Of Reactive Brilliant Blue (KN-R) By Rhodocyclus Gelatinosus

Reactive Brilliant Blue (KN-R) is an anthraquinone-type dye, which has a wide-ranging application and a high output. The wastewater from manufacturing and processing operations is imbued with high-color, big-toxicity and poor biodegradation. As a result, decolorization of Reactive Brilliant Blue (KN-R) is essential. In this paper it is found for the first time that, through the separating and sorting of microorganizism, a strain Rhodocyclus gelatinosus is capable of decolorizing Reactive Brilliant Blue (KN-R), the percentage of decolorization being over 93% under the optimal conditions.Separated and sorted from textile wastewater, the bacteria strain can grow under both anaerobic and aerobic conditions. It is identified as Rhodocyclus gelatinosus and named temporarily XL-1. The optimal growth conditions are as follows: intensity of illumination is 20001x; temperature is 30癈 and pH is 7.0. The bacteria strain contains bacterial chlorophyll a and carotenoid and can photosynthesize.Decolorization of KN-R by Rhodocyclus gelatinosus is carried out under anaerobic condition , in which the optimal decolorization conditions are as follows: temperature 30癈, pH 7.0, and intensity of illumination 2000 1x.The mechanism in which bacteria XL-1 decolorize KN-R is cometabolism. Cometabolic substrate can be peptone, beef extract, glucose, citric acid, malic acid, starch and sodium acetate, among which peptone is the best substrate of this study and the optimal concentration of substrate is 4g/L.Different Immol/L metal compounds have different effects on activity of decolorization of KN-R. MgS04and MnSOi can increase the activity of decolorization of KN-R while some other compounds have inhibitory effects on the activity of decolorization of KN-R, theAbstractsequence of the inhibitory effect of metal compounds on KN-R decolorization being as follows:AgN03>CuS04>HgCl2>Zn SO<>Co (N03) 2>Pb (N03) 2.After studying bacteria XL-1' s decolorization of different initial concentrations of KN-R, decolorization kinetic equation is established.Under the optimal conditions of KN-R decolorization, bacteria XL-1 can decolorize Hostlam Blue R , Bromamine acid , Reactive Brilliant Blue (XNR)and Alizarin Brilliant green GS whose concentrations are 50mg/L, their percentage of decolorization being 77. 7%, 73. 6%, 39.32% and 2% respectively.The decolorization enzyme can be extracted from Rhodocyclus gelatinosus XL-1 and its characteristics are studied. The optimal conditions are: temperature 30癈 , and pH 7.0. The decolorization enzyme can be stabilized below the temperature of 40癈 and within the range of 6.0-8.0. Oxygen inhibits the activity of the decolorization enzyme.Rhodocyclus gelatinosus XL-1 harbours a plasmid coding for biodecolorizative enzyme, its size being about 23kb. After plasmid is eradicated, the ability to decolorize also disappears. Therefore, it is proved that decolorization enzyme gene is located in plasmid.This paper proved that Rhodocyclus gelatinosus XL-1 can decolorize KN-R, and provide the foundation for construction of gene-engineering bacteria.