Extraction And Purification Of Sanggenon C And D

The identification, pharmacology and chemical constitutes of SangBaiPi(root bark of morus), and the pharmacology, physical chemistry properties, chemical structure, analysis and separation method of Sanggenon C and D were reviewed. The content of Sanggenon C and D in SangBaiPi were determined as 2.429 and 2.955mg/g root bark respectively. The new separation process of Sanggenon C and D and the operation parameters was established.The new process is composed of three parts: leaching, extraction and column chromatography.In leaching operation, based on the investigation of the effect of solvent ( methanol, ethanol, acetone, ether and ethyl ester) on the yield and content of Sanggenon C and D of the extract, acetone was the best solvent selected. The particle size distribution of SangBaiPi powder, the equilibrium constants of Sanggenon C and D between acetone solution and root bark powder, leaching dynamic curves, and the internal diffusion coffecients of Sanggenon C and D were determined. At the while, the relationship between solvent to solid ratio, leaching stage numbers and yield of Sanggenon C and D by batch operation was achieved based on an equilibrium model. As a result, the optimal operation parameters were as follow: solvent to solid ratio was 4.0 mg/g root bark, leaching stage numbers was 2, leaching time was between 10 minutes and 20 minutes. Furthermore, percolation was studied as well. The results showed that percolation was better than leaching judging by solvent consumption and productivity. The percolation model taking into account of internal diffusion, external diffusion and nonlinear equilibrium equation was established.In extraction operation, the effect of solvent, solvent to solid ratio and extraction stadge numbers on loss of Sanggenon C and D and removal of impurity was studied. In column chromatography, the effect of different solid phases including active alumina, silica, polyamide, gel, ODS and phenyl silica on separation of Sanggenon C and D was researched, the results showed silica and polyamide were good solid phases for separation of Sanggenon C and D. Furthermore, the elution solvent, elution method, column density, sample capacity were studied in-depth. Sanggenon C and D could be extracted from root bark by using base becauseSanggenon C and D are acidic, but Sanggenon C and D can be degraded in strong base solution. In order to find out the feasibility of extration by base solution, the stability of Sanggenon C and D in 0.5%Na2CO3, 2.0%Na2CO3, 3.5%Na2CO3, 5.0%Na2CO3, 0.5%NaOH, 1.0%NaOH, 1.5%NaOH, 2.0%NaOH solution was investigated, and the degradation rate equations of Sanggenon C and D were as follow:1) In NaOH solutiondC/dt = -1.308 × 1011exp(-77702.8/RT) CC1.401 for Sanggenon C,dC/dt = -5.751 × l0111exp(-83037.4/RT)CC1.400 for Sanggenon D.2) In Na2CO3 solutiondC/dt = -1.414 × 1015 exp(-102918.5/RT)CC0.2955 for Sanggenon C,dC/dt = -6.163 × 1014 exp(-101951.3/RT)CC0.3081 for Sanggenon D.The results showed that the lossity of Sanggenon C and D were below 5.0% in Na2CO3 solution concentration under 2.0% and NaOH solution concentration under 0.5% at room temperature if reaction time was shorter than 30 minutes. Furthermore, the effect of base, consumption, reaction time, extaction stadge numbers, pH on yield of Sanggenon C and D was researched.