| Electrodes are available experimental platform to study the electron transfer behaviors of biomolecules. Based on the specific interactions among the biomolecules, the bio-affinity electrodes are fabricated and applied in bioelectrochemistry. It will be of great help for bioassay, further understanding the relationship between the biological function of some biomolecules and their structures. Besides, it may also be important significance to enrich the theory and techniques in the filed of chemically modified electrodes. In the present dissertation, appropriate and modified electrodes are employed to analysis biomolecules. Self-assembled technique and chemical adsorption approaches are employed to modify graphite and gold electrodes, the combination of electrochemical studies with spectroscopic measurements on the interactions between small molecules and DNA, such as thionine, dopamine, and emodin etc.. Electrochemical impedance methods can be used as an effective method for the interactions between DNA and small molecules. Nanosized materials modified graphite electrodes exert excellent selectivity and sensitive in analysis neural molecules, especially, Nafion-SnO2 modified graphite electrodes has electrochemical recognition of NO molecules, the concentration of NO in rabbit's brain is detected. Antibody proteins were immobilized on cysteamine self-assembled gold electrode by silver colloid. The antigen of Interleukine-2 is detected. The analysis of the electrochemical impedance behavior of such layers can lead to a sensitive immunosensor for the direct detection of the antigen/antibody interaction. An obvious electrochemical behavior was obtained when a gold electrode assembled gelatin by putting it into gelatinase solutions, including medium culturing cancer cells. The results prove the brilliant future for the development of a rapid electrochemical method on gelatinase analysis.
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