Research Of Effective Substances In The Prescription Compatibility Of Refined Xuefuzhuyu

Prescriptions are the main form of traditional Chinese medicines (TCMs). Theory of compatibility is the essential part of TCM prescriptions. In order to develop the advantages of TCM, it is necessary to clarify the effective substances of TCM by means of modern science and technology. In this paper, the refined Xuefuzhuyu (XFZHY) prescription was taken as an example to study the chemical changes of all components, pharmacological changes and the effective compounds in the compatibility. It laid a foundation for the modernization of TCMs.In this paper, the comprehensive design of studying overall chemical components in TCM compatibility was put forward for the first time. For the complex system as herbs, a good separation method was developed firstly, which included reproducibility, proper resolution and retention times. Separation mode, column, mobile phases and operating parameters were optimized to reach this goal. Then, information of retention time, ultraviolet spectra (UV) and molecular weight were used to confirm the origin of peaks in co-decoction. Finally, Chemical changes of components in TCM compatibility were compared. On the basis of this study, three herb couples and one herb team in refined XFZHY under different extraction conditions were studied to find their chemical changes. The results indicated that some components changed in content after compatibly mixing. Whereas, only one regenerate component was found in the ethanol extracts of Ligusticum wallichii, Radices paeoniae rubra herb couple and the water extracts of Bupleurum falcatom L, Radices paeoniae rubra herb couple.In order to study the compatibility in pharmacology, high-fat model of rats was used to investigate the effects of refined XFZHY prescription, different herb couples and herb teams to different pharmacological indexes, which include blood fat,platelet function, leukocyte activity, blood viscosity, vaso-active substrate and akaryocyte function. The effects of different extracts of refined XFZHY prescription and its different herb couples to blood fat, platelet function and vaso-active substrate were also studied. The pharmacological results indicated that these herbs could act on different aspects of arteriosclerosis. The curative effects after compatibly mixing were enhanced to some aspects. Effects of different extracts were not exactly the same. Furthermore, different extracts of effective compounds were investigated on cell. Three effective compounds could protect the damage induced by Ox-LDl and two of them could improve the contents of NO remarkably.Based on the pharmacological study, for the first time HPLC-MS/MS was used to study multiple components in rat serum after administrating different herb couples and herb teams in the XFZHY. Some components in rat serum were qualitatively analysed, while three flavonoids were quantitatively analysed. HPLC-MS/MS was also applied for the qualitative analysis of glycoside components in the extracts of Radix paeonia rubra (RPR) and a total of 21 glycosides were identified. It is indicated that both the number and contents of components in RPR were higher than those of RPA by using HPLC-MS/MS for studying differences between Radices paeoniae alba (RPA) and Radices paeoniae rubra (RPR). The extracts of effective compounds were investigated by HPLC-MS/MS. To some extent, the effective substances of prescription compatibility were clarified.