| Polymeric separation media having a of high selectivity, chemical stability in the entire pH range, good hydrophilicity, high column loading, are more suitable for biopolymer separation, and will be the tendency for the development of stationary phase in the future. With the combining dispersion polymerization with swelling polymerization and polymeric solution porogens, this thesis presents monodisperse poly(glycidylmethacrylate-ethylenedimethacrylate) (POMA/EDMA) particles with macroporous synthesized by one-step swelling and polymerization method. Based on this media, a series of new stationary phase for HPLC were synthesized by new chemically modified methods and can be applied for biopolymer separation. The thesis includes the following eight parts:1. Chapter 1 provides a comprehensive review on the recent development of the polymeric matrix stationary phases in HPLC, mainly on polymeric matrix types, preparation methods and chemically modified methods. The tendency for separating biopolymer by polymeric matrix was also predicted. Totally 150 references were listed.2. Adopting dispersion polymerization method, monodisperse polystryrene seed beads were prepared. The effects of the concentration of monomer, amount of initiator, amount of stabilizer, reaction temperature and time on particle distribution and diameter were systematically investigated. Monodisperse, polystryrene seed beads with low molecular weight in the range of 1.5-7.0 m were prepared under optimized reaction conditions.3. Taking polystryrene beads of 3.4 m as seed, mondisperse PGMA/EDMA beads with macroporous in the range of 8.0~12.0 m were prepared by a single-step swelling and polymerization method. The effects of the concentration of monomer, small molecular and linear polystyrene on pore size distribution in uniformly sized porous PGMA/EDMAbeads were firstly investigated in details by gel permeation chromatographic method, and a kind of PGMA/EDMA bead with suitable pore size distribution range was picked out for separation of five standard polystyrenesamples by size exclusion chromatography(SEC).4. Using PGMA/EDMA beads with suitable pore size distribution range as matrix, a series of hydrophilic modified reactions were carried out for the first time by using small molecular compounds, such as glycerol et al. A kind of modified resin with better hydrophilicity was used for the separation of three proteins and a peptide by SEC. It was found that it followed SEC mechanism and a satisfactory result was obtained.5. Based on PGMA/EDMA beads with macroporous PGMA/EDMA as matrix, three kinds of resins, as weak cation exchange (WCX), strong cation exchange (SCX), middle-strong cation exchange (MCX) chromatographic stationary phase were synthesized by new chemically modified methods. Each kind of stationary phases was evaluated with the chromatographic behaviour, isolation, reproducibility, hydrophilicity, effects of salt concentration, salt type, column loading and pH on the separation and retention of proteins. It was found that the three kinds of stationary phases follow ion exchange chromatographic (IEC) retention mechanism, and can be used for rapid separation of biotechnology production with satisfactory results.6. Using PGMA/EDMA beads with macroporous PGMA/EDMA as matrix, three kinds of weak anion exchange (WAX) packings and two kinds of strong anion exchange (SAX) chromatographic packings were synthesized by new chemically modified methods. The difference in terms of resolution of the packings were explained according to hydrophilically modified methods and mass recoveries of proteins. The chromatographic behaviours of WAX and SAX packings were proved to be excellent and were applied for the separation of recombinant human stem cell factor (rhSCF) and granulocyte colony stimulating factor (rhG-CFS).7. Applying PGMA/EDMA beads with macroporous PGMA/EDMA as matrix, two new kinds of hydrophobic interaction chromatographic (HIC) stationary phases with diol and...
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