Intercalation And Assembly Of Supermolecular Immobilized Penicillin G Acylase

The properties of Layered solids have been reviewed with an emphasis on layered double hydroxides (LDHs) and α -zirconium phosphate ( α -ZrP). The two layered materials were used as supports for the immobilization of penicillin G acylase (PGA), which is an important enzyme for catalysis of the deacylation of penicillin to afford a necessary intermediate in the manufacture of semisynthetic penicillins. The immobilization of PGA couldn't make a progress due to the unfittable supports. In this paper, 1) adjustment and control of the structure and properties of supermolecular materials and construction of molecular reactors; 2) immobilization of PGA in molecular reactors.1. Adjustment and control of the structures and properties of the supermolecular materialsTaking advantage of their intercalation properties which allowed assembly of host materials in the interlayer region, the optimization and control of the structures, properties and surface characteristics of the supports were successfully completed.1) Highly crystalline layered double hydroxides were prepared by adjusting the identity of the di- and tri- value metal cations and their ratio.2) Pillared layered materials with guest molecules were prepared using a novel method and their modified surfaces allowed immobilization of PGA.3) The specific surface area and base strength of layered double oxides (LDO) were adjusted and controlled by changing the calcination temperature, adjusting layered sheet composition and electrical density of the precursors.4) Guests with different functional groups were intercalated into the layers of α -ZrP, thus facilitating immobilization of PGA, and the structures of pillared layered materials were investigated in detail.2. Studies of supermolecular PGA pillared materialsThe structures of PGA immobilized on pillared layered materials were investigated.The ability to adjust and control the structure and pore size of the layered materials, together with the high specific surface areas and memory effect of LDO allowed successfully immobilization of PGA.1) PGA immobilized on glutamate pillared LDHs had a high expressed activity and excellent thermal and operational stability. PGA was shown to be intercalated into the layers of LDH.2) PGA immobilized on LDO has high expressed activity and good thermal and operational stability. PGA was shown to be adsorbed on the surface of LDO.3) PGA immobilized on modified -ZrP has high expressed activity and good operational stability. PGA was shown to be adsorbed on the surface of layered sheets of -ZrP.