Studies On The Applications Of Microemulsion Systems In TCM Nanocarrier Drugs Preparation And In Illicit Drugs Separation

Microemulsion technology has caused more and more attentions as its potentialversatile applications in many research fields recently, especially in the nanomaterialsynthesis and separation science etc. In the present dissertation, the study focuses onthe applications of microemulsions in the preparation of TCM nanocarrier drugs(NCD) and rapid separation of illicit drugs. The dissertation work included two parts:Part I: Studies on the preparation of TCM NCD through the microemulsionin-situ polymerization (MEP) method. Due to the TCM NCD preparations couldimprove the administration efficiency of TCM, the microemulsion in-situpolymerization techniques were developed to synthesize different lipophilic orlipophobic TCM NCDs with different nanomaterials for the purposes of promotingthe modernization of TCM preparation forms, meanwhile, the synthesis and releasingmechanisms of TCM NCD were also investigated. The main achievements obtainedin this study include:① The PMA nanocarrier and aminoorganosilica nanocarrier particles withcontrolled diameters less than 100nm were successfully prepared through O/W andW/O MEP technique respectively, and thus confirmed that MEP is adaptable to thepreparations of both organic and inorganic nanocarriers. Furthermore, the size ofnanocarrier particles had been successfully controlled by the means of step feedmonomers (SFM) in MEP, meanwhile, the synthesis scale had been magnified by 4times by using the SFM technique, verifying the industrialization possibility of theproduce of NCD through MEP.② A PMA NCD of lipophilic breviscapines (a cardiovascular TCM drug), aPMA NCD of colchicines (an anti-cancer TCM drug) and an organosilica NCD ofbrevicapines were prepared by O/W or W/O in-situ MEP, and their drugencapsulation percentages (EN%) reached 90%, 70% and 30% respectively. Abovethree examples proved the feasibility that the in-situ MEP method is suitable tosynthesize different TCM NCDs with different lipophilic or lipophobic properties.③ The investigations on the drug loading mechanism and the drug releasingdynamic mechanism revealed that the synthesized TCM NCDs were of encapsulationtype, and their drug releasing mechanism was well accordant to Higuchi type, a drugreleasing way through framework confusion.Part II: Studies on the application of Microemulsion Capillary Electrophoresis(MEEKC) in the origin determination of illicit drugs. Here, the use of MEEKC torapidly separate heroin and amphetamine drugs as well as the separation mechanismwere investigated in detail. Meanwhile, the pattern recognition of MEEKC fingerprintwas applied to conduct the origin determination of illicit drugs. The mainachievements obtained include:④ The rapid separations of both heroin and amphetamine were well performed,in concrete, 16 species of heroin and its impurities, adulterants and dilutants wereseparated within 10min, and 9 amphetamine drugs and related ephedrine alkaloidenantiomers were also separated within 10min. Moreover, the research results on theseparation mechanism showed that the oil phase inside microemulsions was theprincipal factor to make MEEKC separate all different basic, acidic and neutralheroin compounds rapidly.⑤ The pattern recognition of MEEKC fingerprint was applied to the origindetermination of illicit drugs for the first time, and the fingerprint pattern recognitionof Modified Nei Coefficient Mode only depending heroin MEEKCelectrophoregrams was successfully used in the classification of real heroin seizures,and thus provides a convenient method to the heroin origin determination.