Preparation Of Novel HPLC Molecular Imprinting Chiral Stationary Phase

Molecular imprinting, a novel technology mimicking the 'lock-to-key' phenomenon which happens between antigen and antibody when they interact in biology, is performed by polymer with 'predetermined' molecule recognition to the imprinted molecule, it has found wide applications in areas such as chiral separation, pharmaceuticals assay, biomimic sensors , catalysis and so on. Although the molecular recognition ability of the molecular imprinting polymer was affected by several factors such as functional monomer, cross-linker, porogenic solvent and initiation method, the interaction between the imprinted molecule and functional monomer was the determinative factor. In this thesis, by using amino acid derivatives as imprinted molecules, the effect of functional monomers and cross-linker to the chiral separation ability of the molecular imprinting polymers was studied systematically. Molecular imprinting high-performance liquid chromatography stationary phase was also prepared by in situ polymerization to overcome the disadvantage of preparation of molecular imprinting chiral stationary phase by bulky polymerization method.In chapter one, the original source and recent development of molecular imprinting technology were reviewed. Much attention was paid on those factors such as the functional monomer, cross-linker, porogenic solvent, and initiation method, which involved in the preparation of noncovalent molecular imprinting polymers. Applications of molecular imprinting polymers in chiral separation, Pharmaceuticals assay, biomimic sensors, catalysis and organic synthesis were also introduced.In chapter two, molecular imprinting polymers against amino acid derivatives were prepared utilizing 2-vinylpyridine (2-VP) as basic functional monomer. It was found that there was compatibleness between the functional monomer and imprinted molecules. The stronger the noncovalent interaction between the functional monomer and the chemical functional groups linked to the chiral center in the imprinted molecules, the more stable the complex of the functional monomer and the imprinted molecule, the more possible to keep the complex configuration in the polymerization process, the stronger recognition ability of the imprinting...