Chromatographic Separation In Electrokinetic Analysis System

Based on the principle of electroosmotic pump delivery, chromatographic separation, UV detection and flow analysis, an electrokinetic flow analysis system was presented for reversed-phase chromatography separation. In this dissertation, the electrokinetic delivery theory and experimental techniques of the flow analysis system were investigated and studied, and the flow analysis system was applied to the chromatographic separations preliminarily.A porous glass core electroosmosis pump with moderate pressure was studied and fabricated. Theory expressions of the flow rate and maximum output pressure of the electroosmotic pump were deduced, and validated by the experiment results. Hexamethylene tetraamine was used as an additive of the pump carrier to obtain a stable flow rate and high delivery efficiency. The influences of hexamethylene tetraamine concentration on the flow rate and delivery efficiency of the pump were studied. When 0.05 mmol/L hexamethylene tetraamine was used as the pump carrier, the effect of some variables relating to the electric field, pump structure and carrier component on the pump flow rate and maximum output pressure was investigated in detail, including the electric field strength, the glass porous core length, diameter and sintering temperature, the organic solvent and concentration in the carrier solution etc. A moderate-pressure electroosmosis pump with its flow rate from μL/min to mL/min and output pressure of 1.1 MPa was fabricated, which can be used as the delivery pump for the electrokinetic flow analysis system.Based on the sol-gel polycondensation method, silica monolithic columns of mm diameter, which can be used as a separation column bed, were successfully prepared...