Study On Chemometrics Resolution Methods And Their Applications For The Analysis Of Hyphenated Chromatographic Data

The modern analytical instruments, especially chromatography and hyphenated chromatography, can provide abundant measurement information of multi-component in complex mixtures. They create favorable conditions for the solution of analytical problems arising from modernization of Chinese herbal medicines (CHMs), study of system biology, environmental science, and others. On the other hand, many multivariate resolution methods developed in Chemometric subject, made the effective extraction of qualitative and quantitative information of chemical components existing in hyphenated chromatography with high complexity be possible. In the study of CHMs, the quintessence of our country, it is noticeable that elucidation of materials basis is the first and essential step for the discovery of bioactive ingredients, interpretation of pharmacological experiments, quality control of medicinal materials and the final products, mechanisms of compatible effects of multi-component in CHMs and so on. This thesis is based on the solution of these practical problems. It focuses on the development of novel Chemometric resolution algorithms to high dimensional chromatographic data and the settlement of difficult problems in the analysis of multi-component in CHMs and biology research. Mainly, the thesis comes down to three aspects as follows: 1), resolution algorithms to hyphenated chromatographic data and their real applications in the study of CHMs; 2), discovery of biomarker patterns using Chemometric methods; 3), application of chromatographic...