Studies On Molecular Probes Detection Of Harmful Algae And Its Application

Harmful Algal Blooms (HABs) Harmful Algal Blooms (HABs) are increasing globally. Aimed directly at rapid classification and identification of HABs species, this dissertation developed the essential methods of classification and identification of HABs species, and evaluated new methods for rapidly identifying and classifying HABs species using morphology, molecular taxonomy, chemotaxonomy and in situ growth rate methods. Meanwhile, series of studies were also undertaken to design, screen, and test the molecular probes, including lectin, oligonucleotide and peptide nucleotide acid probes. Corresponding to the three probes described above, the application protocols were evaluated both in the laboratory and in the field.. The main results are as follows:1. The methods used for collecting samples and establishing specific HAB cultures are described in detail. Methods of SEM (scanning electron microscope) and ESEM (environmental scanning electron microscope) for Gymnodinium were developed and optimized. MultisizerTM 3 Coulter counter was found to be accurate, and more reliable for HABs cell enumeration. The responses of phytoplankton under different nutrient limitation depended on their groups. It was possible to use fluorescence intensity for indicating algal physiological status and nutrient variations in batch cultures, however, different results might be given by using HPLC and in-vivo fluorescence protocols.2. Chemotaxonomy based on pigment analysis could be used to identify HABs species belonging to different genera and also different strains belonging to the same genera and species. It also could provide more information for identifying and...