Research Of Polyphasic Taxonomy And Integrated Control To Sulfate-reducing Bacteria

Sulfate-reducing bacteria(SRB) is one of very important technique bacterium by researched. In particular, SRB corrode to metal, people had reported about its corrosion mechanism and control measurement. But corrosion hadn't been resolved to water, crude oil and gas transmission pipeline at all time. In the article, SRB were enrichment, detachment and purification coming from difference recirculated water, and four strains had been came into possession. The polyphasic taxonomy of four strains was researched, including traditional sorting process, chemical sorting process and molecular biology sorting process.A novel thermophilic, acidityphilic and heterotrophic sulfate-reducing bacteria, strain CW-01, was isolated from The Unite Management Stage in oil-field. The bacterium is an facultative anaerobe, non-motile, Gram-positive, pole flagellum, spore-forming curved rod growing within a pH range of 2.8~11(optimal growth at pH5~6.5) and temperature range of 15~50℃( optimal growth at 37℃). The optimum NaCl concentration for growth is 0.7%. Strain CW-02, was isolated from ground sewerage in oilfield United Management Station. Facultative anaerobic, optimal growing pH of 2.8 and the tiptop temperature at 48℃, The optimum NaCl concentration for growth is 30.0g/L. CW-03, was isolated from crude oil well whose depth is 4 kilometer The bacterium is an strictly anaerobic, unendureing acid and maximum temperature is 68℃. A limited number of short chain organic compounds are utilized as electron donors. Strain. Two strains are non-motile, curved rod, Gram-positive, pole flagellum, spore-forming.A new thermophilic, acidityphilic, sulfate-reducing heterotrophic bacteria, strain CW-04, was isolated from biofilm dirtiness in pipeline wall after press pump of affusion system in oilfield. The bacterium is an concurrently anaerobic, non-motile, Gram-positive, pole flagellum, spore-forming curved rod growing within a pH range of 3.7~10(optimal growth at pH 6~6.5) and temperature range of 20~63℃( optimal growth at 47℃) , the optimum NaCl concentration for growth is 0.7%. While sulfate and sulfite were used as electron acceptors to four strains, A limited number of compounds are utilized as electron donors: acetate, lactate, pyruvate, butyrate, succinate, malate, fumarate, valerate, caproate, heptanoate, octanoate, decanoate, tridecanoate, pentadecanoate, palmitate, heptadecanoate or ethanol, yeast extract and glucose, fructose as only sources of carbon. The following substrates were not utilized: formate, benzoate undecanoate, dodecanoate and tetradecane. propanol, butanol, H2+CO2 (80/20%; v/v), acetate (1 mM) + H2. While lactate was used as electron donors, sulfite and thiosulfate, but not sulfer or nitrate, can be used as electron acceptors.Analysis of the 16s rDNA sequence allowed four strains, CW-01(accession numbers: AY742958) CW-02(accession numbers: AY703031) CW-04(accession numbers: AY703033) to be classified as a representative of a new strain of the genus Desulfotomaculum, Desulfotomaculum aeronauticumd(Bacteria, Firmicutes, Clostridia, Clostridiales, Peptococcaceae ) whose similarity is 99%. CW-03(accession numbers:AY703032)as new strains of the genus Desulfotomaculum, Desulfotomaculum nigrificans and Desulfotomaculum aeronauticumd (Bacteria, Firmicutes, Clostridia, Clostridiales, Peptococcaceae ) with BILST on line whose similarity is 98%. But CW-03 belongs to Archaea by means of multiple sequence alignments, and it should belong to Archaeoglobus.SRB's growth curve, anti-chemical of 1227 and corrupted velocity had been measured.Furthermore, a set of systems approach was obtained to inhibit SRB in recirculated water in oil-field: three multifunctional chemistry were matched, several species were selected and physical device was used in order to control SRB. Measurement was summarized to apply in oil-field based on findings.