Hydrogen Production By Anaerobic Fermentation From Biomass After High Efficient Hydrolysis

Hydrogen, as a clean energy that can replace conventional fossil fuels, is attracting worldwide attentions. In this paper, a detail research of hydrogen production by anaerobic fermentation from straw and hyacinth was carried out.First of all, rice straw was degraded by commercial cellulase. Effect of various process parameters, such as pretreatment methods, reaction time, and hydrolysis temperature and pH values was examined with respect to saccharification rate to determine the optimum of hydrolysis parameters.Bio-Hydrogen production by combination of Supercritical Water hydrolysis and anaerobic fermentation was proposed. Limited by the experimental apparatus, rice straw was degraded in Near Critical Water. Saccharification rate of rice straw reached to 50.41 % at 280°C and 20Mpa. The sugar components of the liquid products were analyzed by HPLC with the KS-802 sugars column. Other small molecule organic products were detected by GC-MS analysis, as showed that there were some polarity components with methyl, ethyl, methylene and hydroxyl, etc. The experimental data was simulated by heterogeneous first-order reactions model and the experimental date was coincided with the model.Experiments were carried out to show the effects of pretreatment methods, different mixed bacteria and different inoculation methods on fermentative hydrogen production by rice straw. And hydrogen production by Enterobacter aerogenes and mixed bacteria from different sugars was also investigated. The influence of the main parameters such as particle size, the ratio of substrate to cellulase, pretreatment method and pH value on the straw fermentation was investigated Gompertz equation was used to simulated the dynamics curve of hydrogen production.Hydrogen from the fermentation of hyacinth by digested sludge is demonstrated. The leaf and root of the hyacinth showed different hydrogen production potential because of the diverse metals content in different parts of the hyacinth. And a further study was carried out to investigate effects of different metal ion and its concentration on hydrogen production by Enterobacter aerogenes from glucose. Finally, hydrogen production by hyacinth was carried out in a fermenter with a volume of 5L.Fe-hydrogenase coded gene from Enterobacter cloacae was express by vector in non-hydrogen producing Escherichia coli BL21. And fermentative hydrogen production was carried out in batch experiment using recombinant E.coli.