| Dynamic Analyses of Protein and Growth Factors, Polymorphism and Identification of High Molecular Weight Protein in Sow Milk were examined in the present paper, involving following aspects:1 Dynamic Analyses of Lactose, Protein, IGF-I, EFG and Insulin in Erbualian Sow Milk and Their Comparisons with Yorkshire SowsWith Experiment 1 and LI, lactose, protein and growth factors (IGF-I, EGF and insulin) in Erhualian and Yorkshire sow milk were characterized during lactation (I? Id) after parturition. meanwhile, milk fat was determined in day I arid 6? postpartum in Experiment 11. Lactose concentrations in Erhualian and Yorkshire sows were lower in colostrum. then gradually increased as lactation progressed. Lactose concentration was much lower lot?Erhualian vs. Yorkshire SOWS in day 0-4. & 7 and 8梚t) postpartum (P < 0.05 or P < 0.01), and trended to he lower in oilier lactation period, but no significant. Milk protein levels in Erhualian and Yorkshire sows were highest on day I postpartum, then sharply decreased with a week, hut varied little in day 8 ? 1. However, the significant difference was not observed in the levels of milk protein between Erhualian and97Yorkshire sows (P > 0.05). Lactose and protein in sow milk were negatively correlative, and correlation coefficient (r) in Erhualian and Yorkshire sows was?.703(P < 0.01) and ?.577 (P < 0.01) respectively. Milk fat concentration in Erhualian sows was significantly higher than that in Yorkshire sows in day I and 6-.~7 postpartum (P < 0.05).IGF-I concentrations in ErhuaLian and Yorkshire sow milk were highest on day I postpartum, then sharply decreased within a week, but changed little in day 7-~~~21. Compared with Yorkshire sows, milk IGF-I concentration in Erhualian sows was slightly lower during 7 days postpartum, then a litter higher. However, their difference was no significant (P>O.05). Changes of EGF concentration were similar to that of IGF-1 in Erhualian and Yorkshire sow milk during lactation (1-21d). Compared with Yorkshire sows, EGF concentration in Erhualian sow milk was significantly lower on day I postpartum (P<0.05), but similar in other lactation period. Insulin concentrations in Erhualian and Yorkshire were highest on day 1 postpartum, then quickly declined during 4 days, but mutated slightly in other lactation period. Insulin concentration of milk was lower for Erhualian vs. Yorkshire sows in whole lactation period (l?1d), especially in day 0梚, &?, 8?0 and 11?4 such difference got significant (P<0.05).2 Dynamic Analyses of Protein Components in Erhualian Sow MilkThe components of milk protein in Erhualian sows were characterized during lactation (1?0d) after parturition by SDS-PAGE. Relative proportions and concentrations of main proteins were determined. The proportion of a -La gradually increased as lactation progressed, its concentration tended to increase during lactation I?d postpartum, both of which were no significant changes by F test. Except for much higher concentration at 1 d after farrowing, the proportion and concentration of ~ -Lg varied little during lactation (i?0d). tCN was the major component found in mature milk protein. The proportion and concentration of tCN were significantly different during Lactation day (1?0d) after parturition by F test. The proportion of tCN successively increased with the experimental98period, peaked at 20d postpartum. The concentration of tCN was highest at the tirst day, lowest in 1 0~- 13 day postpartum, mutated slightly in other lactation period. The proportion and concentration of SA were significantly different during lactation day (1-~-20d) after parturition by F test. The proportion of SA varied little \vithjfl the first week postpartum, then declined slowly. Its concentration was remarkably higher at Id postpartum, then gradually decreased. lg was the major protein component found in colostrum. The proportion and concentration of Ig were significantly different during lactation (l--20d) afte...
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