Studies On Antrycide-resistance Of Trypanosoma Evansi

冯 Surya is lethal disease OfCattk,bllffalo,horse,pig,ShCep,goat,Cffoel,CtC． caused by Tpanosoma evansl In Asia,Africa and America,particular In almost provinces In China．Since antryclde was Introduced from oversea about thirty years ago,It has been used to treat surra ofllvestocks as a main drug．But drug－resistance was eme吧lug In some areas ofChlna．Forguldinghowto correctlyuse ltandhow to control theproduct ofantryclde－resistance,the studywas carried out． For a raPid determination ofantrycldal dose to T evansi,The in vitro growth－ Inhlbltlon assay was used to define the 6(XJCA,HBM。ZJB,JSBI,JSBZ and ZJBZ)line's sensltlvitles to antryclde．ThelrIC；。values were 0．0015if,0．008436, 0刀1550,0．01620,0．05906 and 0刀8260卜s／ml,respectively．The In vlvo curative tCSt,OflthC OthCYhsfld,WSS USCd to dCtCfllllllCthClfCDI00tO slltryCldC．ThClfCDI00 values were 3．sing／kg,5．omg／kg,7．omg／kg,7．ling／kg,11．omg／kg and．omg／kg, respectively The positive correlative relationship,whose relative value was 0．222,"betweenthelr ICS。and CDI口。was shown a curve and the relationship ig was obtained． In orderto understand situations ofantrypclde－resistance ofT evansi,XJCA, GXM,HBM,ZJB,JSBZ,AHB GDBZ,YNB HNB GDH,JSBI and GDBI populations from nine provinces ofChlna,the in vdIDdID growth－l讪lbltlon test was lSCd to dCtCflTllflC thClf SCflSltlVltlCS to antryCldC．ThCIF IC50 V8luCs Were 0．0015if－ 卜g／il,0刀0739－卜g／ml,0·C08436－卜g／ml,0刀1246－卜g／ml,0．0162－pg／ml,0．0164－ Pg／ffil,0＊225－Pg／ml,0＊246－fig／ml,0＊376－Pg／ml,0＊535Pg／ml,0＊5906－Pg／ml and 0．O6694卜g／ml,respectlvdy The mice In允cted with＊JC人 HBM,ZJB and JSBI lines were treated at antrypclde's dose ofs－mg以g．Their curative ratios were 100％,100％,90％and 60％,in turn．The results indicated that the ZJB,JSBZ, ． AHB,GDBZ,YNB,HNB,GDH,JSB and GDB lines had produced the different levels of＊the drug－resistance,but the XJCA,GXM and HBM Imes．Their referable 121 curative dose(CD；。),from the curve according to their IC。。,In these areas were 2．6mg／kg for XJCA,4．sing／kg for GXM,5刀mg／kg for HBM,7刀mg／kg for ZJB, 7．ling／kg for JSBZ,7．ling／kg for AHB,8刀mg／kg for GDBZ,8．4mg／kg for YNB, 9．6mg／kg for HNB．sing／kg for GDH,11．omg／kg for JSB and．gmg／kg foreGDBZ,respect1VVIy A clone ofT evansi,C01,from Zhqfang bufafalo strain was sensitive to 肛血yclde．lypanosomes ofC01 developed Into seven levels ofantryclde－ resistance lines,CI－C7,t帅ugh,3,6,9,15,22 and 28 lmmunosupressed mice(cycloposphamlde)treated with antryclde at subcuratlve dose by．p andtwo sensitive lines,C02 and C03,tkough 5 and 28 lmmunospressed mice(cycloposphaxnlde)not contacting with drug．The in vitro growth－inhibition sS8y WSS USCdtO dCtCrtTllflC thClf SCllsltlVltlCS to ntryCldC．ThC IC50 V8lllCS OfthC C01,C02 C03,CI,CZ,C3,C4,CS,C6 and C7 were 0刀1550,0．01687,0刀8260, 0．10237,1．40929,1．92290,9．92330,23．56300 and 43．84000卜g／ml,respectively The results showed that the relationship between used total qu咖files ofantryclde ld IC50 WSS 8 CUfVC pstteffi,iCalllflg SIOW fslSC OfthC fCSIStallCC to ltfyCldC讪i CI to C4,and then fast raise oflt from C4 to C7．Onthe otherhand,the．relationship between the quan...