| Hypericum sampsonii Hance, a wild perennial, belong to the genus Hypericum from the family Guttifeae, native to China. It has important medicinal value. A great deal of attention has been paid to the genus because its two main active hypericins and flavonids can effectively inhibit depression, central nervous system and virus, and improve immunity. So, The plant was studied systematically in botanical characteristics, phytochemistry and tissue culture. in this paper some worthy results, which filled in the gaps in the fields of the genus, are reported for the first time. These researches provide a significant scientific basis for H. sampsonii. The main results are as follows.1. The four constituents, hypericin, rutin, hyperin and quercetin are obtained from H. sampsonii by systematical abstraction, repeat chromatography and purification. They are identified by spectrology, physics and chemistry. The study lays a solid theoretical foundation for the medicinal herb that widens the phytochemistry of the genus.2. The method developed for quantitative analysis of hypericin in H. sampsonii by HPLC has good reproducibility and accuracy.3. 30 and 40 chemical components have been separated and identifiedby GC/MS and computer from its leaves and fruits respectively. The result shows that its leaves and fruits differ widely in their components, and the leaves contains ocimene that makes up 14.49% of total volatile content of the leaves, but the fruits do not.4. The meta-analysis on the contents indicated that plantlets, seedlings, plants with flowers and flowers of H. sampsonii are similar in content to those of H. perforation. So, it is also a significant medicinal species containing hypericin.5. Its components distribution are related directly to the structures that can be divided into three types, namely secretory nodule, secretory cavity and canal, where the first one contains hyperieins andthe others contain volatile oils. The number of secretory nodule of leaf ( and petal is much more than that in sepal.6. A steady regeneration system for H. sampsonii with tissue culture was established. The callus is easy to be induced, and it propagates rapidly, then, differentiates into buds. The callus with buds can be subcultured highly at intervals of 20~25 days; it has been continuing for two and a half years without vitrification. The callus and node can differentiate into buds directly in a cluster and then grow into plantlets. The survival rate of its transplanting is very high, up to < 92. 5%.7. The traits of dedifferentiation and redifferentiation of theexplants form H. sampsonii show that the calli the stem and leaf usually form buds at its parietal cells. In the presence of auxin, the parenchyma of the leaf gap at the node is initiated firstly, then, develops blastemata that form buds in a cluster on the node without callus. These show that the various parts of the herb differ greatly in cell initiation and organ differentiation.8. The content of the flavonids in H. sampsonii is 4.33%, higher than the common standards, and that is 5.87% in its callus with buds, 1. 54% higher than the former. These verify that its callus \vith buds has the biosynthesis of secondary metabolite, namely flavonids.
|
PDF Full Text Request