Transformation Of Herbicide And Blast Resistant Genes To Rice And Their Inheritance Behavior

The tissue culture system for elite rice (Japonica) varieties (Fengyou 201 and Chaochan 2, high yield, good quality, but blast disease sensitive) cultivated in Jilin province was optimized. A herbicide and a rice blast disease resistant gene were delivered into those elite varieties. Herbicide and blast disease resistant transgenic rice plants were obtained. The blast resistance of transgenic rice plants and the inheritance of foreign gene in the progeny of those transgenic rice plants were analyzed. Results are as follows:1.The tissue cultural system of mature embryos of elite cultivated rice varieties in Jilin province was optimized.1.1 The callus induction and differentiation efficiency of different genotypes is quite different. The callus induction efficiency of Ji 89-48, Chaochan 2, Fengyou 501, and Fengyou 307 could be as high as 86.4, 88.7, 80.8 and 81. 6 percent respectively. And the differentiation efficiency of Ji 89-45, Chaochan 2 and Fengyou 501 could be as high as 50.1, 45,8 and 49.5 percent respectively.1.2 Reducing 2. 4-D concentration to 1 mg/L, adding NAA (1 mg/L) and KT (0. 5 mg/L) in the subculture medium can improve the growth of callus. And more yellowish, grain-shape cmbryogenic calli was obtained.1. 3 Drying treatment of calli for 16h before differentiation could increase plant regeneration efficiency by 20.6-37.2 percent.2. Parameters of Gene Gun (PDS-1000) transformation of the callus derived from mature embryo of elite rice varieties were studied by Gus gene transcient expression.2. 1 When the distance between DNA-gold pellet and the target cells was 9 cm, bombard for 2 times, the transformation efficiency was the highest. The Gus gene transcient expression efficiency was as high as 88.2 percent.2.2 When the distance was fixed, the Gus gene expression efficiency was increased with the increase of bombarded times. When the distance was 9 cm, and bombarded for 1, 2 and 3 times, Gus gene expression efficiency was 30.6, 88. 3 and 90.0 percent respectively.3. A Herbicide resistant gene (bar) has been delivered into rice. And herbicide resistant transgenic rice plants were obtained.Using the erabryogenic calli derived from mature embryo of elite rice varieties (high yield and early maturing) as the explant, several transgenic rice plants were obtained via Biolistic gene delivering method, bar gene had been inserted into rice genome demonstrated by PCR and Southern hybridization. Three transgenic rice plants (D