| The plant of Rhodiola.L has been widely used as traditional herb medicine in china for a long time. Rhodiola.L contains more than 90 species and grows quite widespread all over the mountain areas of northern hemisphere, of which 76 species are present in china, Tianshan Mountain area of Xinjiang is one of main distribution area of this plant in China. Its extracts from roots possess the effects of anti-viral, anti-tumur, anti-aging, anti-oxygen deficiency, anti-fatigue and other pharmacological properties. It had developed many kinds of health drink that content Rhodiola. L. According to the latest research, this herb medinice posse the function of anti-radition, Planta record of Russian introdued that Astrogation Bureau of Russian used the root of Rhodiola.L to make health drink for astronant that can resist high radiation in outspace, Rhodiola.L has high value of science and application research. So we choosed wild Rhodiola.L as our research material, assessed the plant genetice resource, researched physiology of the seed dormancy and germination, identified of the seed protein RP-HPLC biochemistry marker and so on.In this thesis, the first thing we did was gather, rearrangement, characteristic description and plant morphological photograph for the Rhodiola. L plant that distribute in Tianshan mountain area, Xinjiang China. Salidroside and its precursor tyrosol that are the most important biologically active compounds in dry roots of the Rhodiola L.and heterodendrin that is the main poison compound in this plant were chosen as the main character indicator of the Rhodiola. L. It established a HPLC method to determine the contents of salidroside, tyrosol and heterodendrin; the method is simple, sensitive and accurate. The aim of this investigation was to screen the elite species with higherIVsalidroside and tyrosol contents and lower heterodendrin content in ten species of which are present in Tianshan mountain area. The second thing was that we used combination treatment methods of germination temperature control, a 4癈 pre-chilled treatment and exogenous plant hormones stimulation to research the dormancy and germination porperty of Rhodiola rosea L. seeds, explored the physiology interactions among exogenous stimulation, endogenous plant hormone change and seed dormancy, revealed the physiologic mechanism of seed dormancy and dormancy breaking and got out the best way to break dormancy of Rhodiola rosea L. seeds, to trigger seed germination and to gain the balancing growth seedling. The third thing was that we used RP-HPLC method to separate and identify the Rhodiola.L seed gliadin protein, found the protein biochemistry marker that is species distinct. This work could give technique support of identification to the Rhodiola.L wild species and ento-species bio-diversity research and expand crop inbreeding lines and hybrids purity identificaion measure. Auther summarized research result of this thesis and had gained following conclusions: 1. We found that Rhodiola roseaL. can produce high contents of salidroside and tyrosol and low content of heterodendrin from the result of resourse assess research. The Rhodiola rosea L. grows at lower altitusde and can be obtained more easily compared with other species. There is little difficulty to introduction and domestication this plant. The species is the most valuable Rhodiola.L for further research and utilization in this area.2.We got following conclusions, according to the result of dormancy physiology and induction germination treatment research; (1) Seeds with three weeks in 4癈 prechilled independently had the germination rate of 48?2 % at 25/10癈, more than 3 times (12%) of control group. (2)The seed of Rhodiola rosea L. were soaked with exogenous GAs, 2,4-D and GA3 + 2,4-D for 24 hours and then a three weeks in 4癈 prechilled, the endogenous level of ABA could be decreased in the embryos and be maintained it in a low level, the rate of germination get to 98%, more than 7 times of control group and 1 times of independently prechilled treament.
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