| Based on the previous researches, the synthesis, secretion, the approach of information transfer and activated peptide of the Telenomus theophilae 's kairomone and the components that had immunity and biological activity of kairomone in the procreate system of Bombyx mori female moth were further performed by using the host Theophila mandarina and the replaceable host of T. theophilae, B. mori, as insect materials. Main findings of the study were as follows:1. Detailed studies on kairomone by using the immunolocalization with colloidal gold and transmission electron microscope indicated that the specific labels existed in the secretory cells of endoplasmic reticulum, Golgi complexes, secretory materials, mcrovilli, reservior, branched of reservior, lumen of secretory portion and lumen of reservoir. Combined with the results of ELISA labels in T. mandarina and B.mori, we summarized the synthesis, secrete and information transferring approaches of the T. theophilae-host-recognition kairomone. The kairomone proteins were synthesized in the endoplamic reticulum of the secretory cells that were in the accessory gland of the host and the replaceable host female mothes. After modified in Golgi complex and condensed and transferred by secretory materials, they were absorbed by the microvilli around the reservior. Finally, they got together in the Lumen of reservoir through the Lumen embranchment of the secretory cells and the lumen of the secretory portion. When the female moth laid eggs, they would be secreted to the mid-ovariole and were excreted out of the body with the host eggs by overlaying them.2. Telenomus theophilae host-recognition kairomone hydrolyzed by chymotrypsin contained two parts; they were crystalline (precipitation) and amorphous (supernatant) regions respectively. When hydrolyzed, the crystalline region from B.mori were cleaved into 4 bands of 8.8 kD,9.5 kD,12.8 kD and14.4kD respectively on SDS-PAGE gel. The case was 9.4 kD, 10.3 kD, 12.6 kD andl4.2kD for the crystalline region from T.mandarina .3. The products of kairomone hydrolyzed by chymotrypsin with ELISA and DIGFA analysis were consistent with those by bioassay. There appeared immunity and biological activity in the crystalline and amorphous regions. Western blotting showed that there existed immunity components in the crystalline and amorphous regions. The immunity components were the functional domain where Telenomus theophilae could found the hosts. The composition of amino acids in the crystalline region from B.mori and T.theophilae was very similar. Both of them were composed of at least six amino acids .They were tyrosine, lysine, glutamic acid, methionine, lysine and serine by scale-up. The molar percentage of tyrosine, lysine and glutamic acid was 52.4, 13.3% and 2.3% respectively for the crystalline region from B.mori, and 53.3%, 22.1%, 9.5% respectively for the crystalline region from T.theophilae.4. The kairomone was separated from the female accessory gland of B. mori and T.mandarina by using 2-D electrophoresis technology. The result showed that the proteins from the two kinds of accessory glands were highly similar. Both of the proteins had 5 spots and were distributed in the upper, mid and lower parts of theseparated gel. The materials with the highest contents of protein were in the upper separated gel with the pH value varied from 3.5 to 6.0. For the sake of the investigation of the constitution and the molecular structure of the kairomone, the perfect separation condition of reversed chromatogram technology was established, by which the raw materials of female accessory gland of B. mori and T. mandarina were separated and purred. A group of hydrophilic components and three groups of hydrophobic tic components were separated from both of the two species. The components which were separated by chromatogram from the accessory gland of B, mori was collected according to time window, and tested with ELISA technology. The result showed that each component had immunity. But the i...
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