| Veined whelk, or Asian whelk Rapana venosa (Valenciennes, 1846) is an economic fishery species of the coastal water of China. Little work has been done on population genetics and molecular phylogeny ofR. venosa. Morphological data were collected and analyzed by quantitive traits in the paper. The population genetics variation were studied using allozyme and mt DNA sequencing method, and 16Sr RNA and COI gene fragment markers were developed and applied in R. venosa genetic structure in this dissertation. Nucleotied sequence data from partial 16S rRNA gene were used as an alternative molecular approach to morphology and can provide valuable information on taxonomic relationships among gastropodas.412 samples of R. venosa of 8 populations collected from Qinghuangdao(QH), Dalian(DL), Dandong(DD), Yantai(YT), Qingdao(QD), Rizhao(RZ), Lianyungang (LY) and zhoushan(ZS), the coastal water of China, by using morphological method. The multiple regression equations were constructed to estimate shell weight and shell length of each population. The results showed that the regression relationship for all the characters used in the study were significant, the correlation coefficients between shell weight to shell length an shell width were significant, also the correlation coefficients between shell length to body whorl length and shell width were significant. The cluster analysis results indicated that all the eight natural populations were divided into two ecotypic populations (inshore and offshore population), which representing two different local populations and coinciding with Zhang(1980).Screening of 31 putative enzyme-coding loci and 5 different kinds of tissues of R. venosa for enzymatic activities using starch gel electrophoretic technique proved that 14 znzymes such as G3PD, SDH, MDH, ME, IDH, XDH, CAT, SOD, HK, EST, ALP,AP, MPI, PGM, were active to R. venosa after being stained. They varied between different tissuses, but exhibited stable and clear bands in muscle and liver tissues, Allozymic electrophoresis was used to investigated the genetic variation in 7 populations of R. venosa collected from Qinghuangdao(QH), Dalian(DL), Dandong (DD), Yantai(YT), Qingdao(QD), Lianyungang (LY) and Zhoushan(ZS) respectively . Samples were routinely examined at 14 enzymes comprising 22 putative allozyme loci. The result revealed higher levers of genetic variability, with the proportion ofpolymorphic loci, P =0.3766o.99, average expected heterozygosities per locus He =0.0808+0.0188, average observed heterozygosities per locus H0 =0.0440+0.0127, and the effective number of alleles ~Ae with values of 4=1.1727 0.0353.Nei' s mean genetic distance (D) and identities (I) ranged from 0.0016 to 0.0191 and 0.9899 to 0.9989. Deduced from the date that none of the 7 populations is capable to evaluated to a new species or subspecies according to Nei' s standard .Part of 16S rRNA gene was amplified with polymerase chain reaction (PCR) and sequenced for 102 individuals, 13 from Qinhuangdao(QH), 14 from Dalian(DL), 15 from Dandong(DD), 15 from Yantai(YT), 18 from Qingdao(QD), 17 from Lianyungang(LY) and 15 from Zhoushan(ZS). The result showed that no nucleotide positions were found to have gaps or insert of base pairs among these individuals, 28 variable positions were observed and all of the individuals were grouped into 37 haplotypes. The position 281 (G^A)and 483 (T-C)in Sllbp of DL, 17CA-G),66 (T-C) and 247 (T-*C) of YT, 128 (T-C) of ZS, 130 (T-G) and 161 (A -*-G) of QH, 431 (T-?C) of DD represented the characters of each population, and can be used as genetic markers.Sequence of cytochrome oxidase subunit I (COI) gene was used to investigate the genetic variation in the above 7 populations of R. venosa in the water of China. Part of COI gene was amplified with PCR and sequenced 71 individuals. The result showed that there were 35 variable nucleotides positions in the 656bp segments of thegene and total 71 sequences were grouped into 33 haplotypes. No gaps or insert of base pairs among these individuals were founded. Var...
|
PDF Full Text Request