| A comparative study on Pinus koraiensis Sieb. et Zucc.and P. prokoraiensis Y. T. Zhao, J. M. Lu et A. G. Gu. from three points of view-structural botany, plant physiological and biological chemistry, and molecular biology was carried out. Observe the structures of the roots, stems and leaves of P. koraiensis and P. prokoraiensis by photomicroscop and electric microscop for structural botany study. Measure chlorophyll contents, photosynthesis, transpirational rate, stomatal conductance, water patential, water use efficiency, peroxidase activities and its isozyme, superoxide dismutase activities and its isozyme, and esterase isozymes of P. koraiensis and P. prokoraiensis for plant physiological and biological chemistry study. Sequence the chloroplast rbcL gene, mitochondrial nad5 gene and nuclear 4CL gene of P. koraiensis and P. prokoraiensis for molecular biology study.The results of the structural botany were followed. The transverse section of hypodermis on the dorsal leaves of the needles only had one-layer cells in P. prokoraiensis, but there were another second discontinuous hypodermis in P. koraiensis. There were spiral thickenings on inner wall of tracheids of P. prokoraiensis, but there weren't in P. koraiensis. The intercellular flange in cuticle superficies interna of Pinus prokoraiensis leaves was superficial undulate and thick but that of Pinus koraiensis leaves was profound undulate and thin.The primary structure of their stems both had one layer epidermis on which there were glandular hairs and needle-shaped hairs. The pith was composed of parenchyma cells contained resin and tannin; cortex was also composed of parenchyma cells contained resin and tannin in which distributing tow circles of resin ducts. Outside resin ducts of P. prokoraiensis' cortex were 39 0.24 um in diameter on average, and inside resin ducts were 63 0.41 u m in diameter on average. Moreover, outside resin ducts of P. koraiensis' cortex were 34 0.21 u m in diameteron average, and inside resin ducts were 55 0.31 um in diameter on average. Cortex of P. koraiensis and P. prokoraiensis contained many resin ducts and resin cells. Their phloems both contained albuminous cells. The annual ring of P. koraiensis s xylem was comparatively regular, but that of P. prokoraiensis s xylem was irregular.The tracheids configuration of early wood of P. koraiensis and P. prokoraiensis were similar. P. prokoraiensis's tracheids end were blunt or tine,and the tracheids were 2.65 0.12mm long, 37.52 + 0.22 u m wide, 2.47 + 0.12um in wall thickness; P. koraiensis's tracheids end were tine and smooth , tracheids were 2.43 + 0.11mm long, 37.12 + 0.23 w m wide, 2.24 +0.09 wm in wall thickness. The tracheids of later wood of P. koraiensis and P. prokoraiensis were all tine. The tracheids of P. prokoraiensis were 2.54 0.08mm long, 36.58+0.21 urn wide, 2.51 + 0.11 m in wall thickness and hade spiral thickenings.The P. koraiensis's tracheids were 2.41+0.06mm long, 36.32+0.24 u m wide, 2.31+0.13 m in wall thickness. The cross field pits of P. koraiensis was window-like and the number was from one to two, pit border was evidence, and pit aperture was mostly rotundity; P. prokoraiensis's cross field pit was window-like and the number was from one to four, pit border was distinct, and pit aperture was ellipse or strip- typed sometimes.Through the cross section of root, it clearly showed that the primary xylem was triarch and there was no thin-walled cells of pith in the center of primary xylem. At the side of three protoxylem bundles, there were resin ducks and the fat-secreting cells are thin-walled. There was no tylosoid in the resin ducks of P.prokoraiensis. The diameter of the resin duck opposite the triarch protoxylem ridge was big, as three times as of that of the resin duck far from the centure of the root. The annual rings were obvious. Through the cross section of root of P. koraiensis, it's easy to find that the primary xylem was pentarch. The diameter of the resin duck opposite the pentarch protoxylem ridge was big, as three times as that of the re...
|
PDF Full Text Request