Characterization And Location Of Main Capsid Protein Of Bombyx Mori Cytoplasmic Polyhedrosis Virus

Cytoplasmic polyhedrosis virus (CPV) which can infect more than twohundred insects belongs to the Cypovirus genus in the Reoviridae family.Bombyx mori cytoplasmic polyhedrosis virus is a model of CPV. It appears asa single-shelled icosahedron with 12 characteristic two-layer spikes. The fullBmCPV particle contains a 10-segmented double-stranded RNA (dsRNA)genome and is composed of five structural proteins as well as severalenzymes including transcriptase and transmethylase.The obtained BmCPV research results of molecular biology and the three-dimensional structure of cryo-electron microscope (cryoEM) reveled that thecharacteristic (belongs to structural protein or nonstructural protein) of proteinp101 encoded by dsRNA segment 5 has not been clarified, and that dsRNAsegment 4 encoded the viral capside protein VP3. The VP3 is one of the mostabundant protein in the structural ones of virus, which may be the candidatefor the 120 large protrusions (LPs) on the outside of the viral capsid shell. Inorder to clarify the characteristic of p101 and confirm if the capsid protein VP3is located on the outside of the virus particle or not, we use the BmCPVChinese strain as the material and sequence the full length of the BmCPVdsRNA segment 4 and 5 by RT-PCR and molecular cloning techniques.Segment 4 consisted of 3263 nucleotides and possessed a long open readingframe (14nt-3190nt) encoding a protein (MW:127kDa) of 1058 amino acids.Segment 5 consisted of 2852 nucleotides and possessed a long open readingframe (71nt-2716nt) encoding a protein (MW:101kDa) of 881 aminoacids.The sequences of dsRNA segment 4 and segment 5 have been...