Genetic Analysis Of Several Yield Components And Yield-related Characters In Japonica Rice And Cloning Of Oen Gene OsZPT3-1 Related To Rice Panicle Development

In this study, several japonica rice landraces from Taihu lake region and one RIL population derived from from the(Jicaiqing × IR26) has been used to analysis the inheritance of yield components and yield-related characters, including total spikelets number per panicle, filled spikelets number per panicle, effective panicle numbers, 1000-grains weight, seed setting rate, panicle length, plant height, heading date and grain density, and to map their QTLs. Through bioinformatics and the RT-PCR approach, a novel zinc finger protein gene, belonging to TFIIIA type, was cloned from Japonica rice landrace Jiucaiqing and designated as OsZPT3-l, which is specifically expressed in the rice panicles, but not detected in stem, leaves, anthers and roots at the adult stage.The results summarized as follows:1. Genetic Analysis of Several Yield-related Characters in Japonica Rice Landraces from Taihu Lake RegionThree crosses derived from five accessions with distinctly different panicle traits, including Datoudao × Daichangqing(cross I ), Laolaihong × Yan Jing-2(cross II ) and Daichangqing × Shanghaiqing(cross III), and their derived generations(F₁, F₂, B₁, B2), were used to analyze the inheritance of yield-related characters of japonica rice landraces from Taihu lake region by the major gene plus polygene mixed inheritance model. The result showed that the optimum model for total spikelets numbers per panicle(TSP) in cross I was concoined with one major gene plus additive-dominant polygene mixed inheritance model, while that for cross II and III was concoined one complete dominant major gene plus polygene mixed inheritance model. The optimum inheritance model of effective panicle number per plant(EPN) in cross II and III was one major gene plus polygene, while that for cross I was two major genes. The genetic model of 1000-grains weight(KGW) in crosses II and I were two major genes plus polygene mixed inheritance model, and one major gene plus polygene for cross III. The optimum model of filled spikelets numbers per panicle(FSP) and seed setting rate(SSR) for three coresses was two major genes model...