The Gene Effect Of ZmC₄Ppc Which Was Transferred To Indica Restorer By MAS And Genetic Analysis Of A Mutant With Resistance To Sheath Blight

â… . The gene effect of ZmC₄Ppc which was transferred to indica restorer by MASAbstract: Because of the reason of high photosynthetic efficiency in C₄ plant existing in the key enzymes of C₄ pathway, not in the'cranz' structure and there being necessary genetic machine to express C₄ gene in C₃ plant, the genes having relationship with C₄ photosynthesis can be expressed in C₃ plant as same as or as much as in C₄ plant and the C₄ enzymes expressed have same function. For example, the key enzyme genes of C₄ pathway in maize have been transformed to rice (C₃ plant) successfully and have been expressed highly effective. On the other hand, the genetic engineering technology was complex if exogenous genes were asked to transform receptor materials wanted. The technology of marker-assisted selection (MAS) and Kitaake with ZmC₄Ppc were used in this study. Markers design, verification, MAS and combining ability etc. were studied and their results were as follows:1. The design of specific and efficient markers. The full-length of intact ZmC₄Ppc was 6781 bp. Its band was not clear and repetition was bad when it was amplified by PCR. The accuracy was decreased if ZmC₄Ppc was selected by MAS. Therefore, the gene sequences in maize and rice genomic sequences were utilized, then to BLASTs and got the specific fragments. The software Primer Premier 5.0 was used and the specific primers were designed. To ZmC₄Ppc, Forward: 5' AAG CAG GGA AGC GAG ACG 3', Reverse: 5' GAT TGC CGC CAG CAG TAG 3'. It was named as MRpc. Site of amplification by MRpc was 970-1280bp in ZmC₄Ppc and size of the product was 311bp. The specific primers of ZmC₄Pdk were designed in the same way: Forward: 5'TTG GAT GAC TTT GGG AAC 3', Reverse: 5' AAG TGT CTT TGC GAG GAA 3'. The specific primers were named as MRpd. Site of amplification by MRpd was 376-626bp in ZmC₄Pdk and the size of the product was 251bp.