Analysis Of Tandem Repeat Sequences In The Genome Of Kuruma Prawn Marsupenaeus Japonicus And The Development And Application Of Microsatellite Markers

In this paper, random genome library of Kuruma prawn Marsupenaeus japonicus was constructed and the lengths of 3395 sequenced clones were between 400 to 1000bp. The distribution and frequencies of microsatellite and minisatellite were analyzed from the 2815 sequences spliced; fifteen polymorphic microsatellite primer pairs were screened from these clones included short tandem repeat. Genetic diversity and genetic differentiation in four wild populations of kuruma prawn were detected by six microsatellite loci. In addition, genetic variations between wild and cultured populations of Kuruma prawn were also investigated using microsatellites. The main results are as follows:Kuruma prawn (Marsupenaeus japonicus) is one of the most important fishery animals in the East and South China Sea. With the rapid and uncontrolled development of shrimp aquaculture industry, concerns about the risk on the lost of genetic diversity and genetic impact to the wild population caused by the escape or release of cultured aquatic organisms into the environment have become the primary focus over the past 30 yrs, following centuries of largely unregulated activity (Ryman 1981; Waples 1991a; Utter 19 Then, it would finally result in a decrease of genetic diversity and an overall decline in the fitness of the wild population Therefore, a study to investigate the genetic diversity of wild geographical populations is very important for conservation of natural resources.1. Analysis of distribution and frequencies of tandem repeat sequences based on the sense of whole genome.We constructed the random genome library, and sequenced 3395 clones which their length was between 400 to 1000bp. From the 2815 sequences, 1206 microsatellites and 487 minisatellites were found in the 1606711bp Kuruma prawn genome sequences. Their cumulative length occupied 5.9% and 3.79% of the total length of DNA sequences, respectively.In the microsatellite sequences, dinucleotide repeats were the most frequent type, accounting for 69.82% of the total number of microsatellite, and trinucleotide repeats...