Studies On Effects Of Fat-Soluble Vitamins On Metabolic Responses And Shell Biomineralization In Abalone, Haliotis Discus Hannai Ino

A series of experiments were conducted to investigate the effects of fat-soluble vitamins on the growth, metabolism and shell biomineralization in abalone (Haliotis discus hanani Ino). Relationships among fat-soluble vitamins, metabolic responses and shell biomineralization were studied. The current studies included the following: (1) Effects of fat-soluble vitamins A, D, E and K on soft body composition, the activities of antioxidant enzymes and fatty acid composition in Haliotis discus hanani. (2) Effect of fat-soluble vitamins A, D, E and K on growth, survival and the activities of immune-related enzymes which includes acid phosphatase (ACP), lysozyme, phenoloxidase (PO), catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and glutathione reductase (GR). (3) Effect of fat-soluble vitamins A, D, E and K on chemical and mineralogical composition in shells, chemical elements in soft body and alkaline activity (AKP) in viscera, muscle and soft body of abalone.Four 240-day growth experiments were conducted in a re-circulated water system according to a single factor design. The levels of fat-soluble vitamins A, D, E and K were as follows respectively. Vitamin A was 0 IU/kg, 1×10~3 IU/kg and 1×10~6 IU/kg; Vitamin D was 0, 5×10~3 and 5×10~5 IU/kg; Vitamin E was 0, 50 and 5000mg/kg; Vitamin K was 0, 10 and 1000 mg/kg. There were 12 kinds of diets in all. Triplicated groups of juvenile abalone (A: initial weight, 0.96 ± 0.02 g; shell length, 17.70 ± 0.06 mm; D: initial weight, 0.81 ± 0.00 g, shell length, 16.42 ± 0.03 mm; E: initial weight, 0.71 ± 0.00 g, shell length, 15.49 ± 0.04 mm; K: initial weight: 1.20 ± 0.01 g; shell length: 19.24 ± 0.01 mm ) of each kind of vitamins were fed satiation one of 3 semi-purified diets as mentioned above. The results are summarized as follows:(1) On 240 days, The activities of SOD and GPX in soft body of abalone fed with 1×103 IU/kg dietary vitamin A were significantly higher than those with 0 IU/kg 和 1×10~6 IU/kg vitamin A supplements (PO.05). The content of C18:1n-9, C18:2n-6 and C22:6n-3 in soft body of abalone with 1×10~3 IU/kg dietary vitamin A supplement were significant higher than the other two groups (P＜0.05); The highest value of C20:4n-6 was found as 7.83 ± 0.24 % in soft body of abalone with 1 ×10~6 IU/kg dietary vitamin A supplement.