| Micro-particle adherenced plasmid pBarGus has been used to transform threeLolium perenne L. varieties; and Agrobacterium tumefaciens encoding NPTH and NAS genes has been used to transform three Agrostis stolonifera L. varieties. By optimization of technologies for turfgrass genetic transformation, establishing a high frequency particle bombardmental or Agrobacterium tumefaciens transformation systems and providing a new technical platform for turfgrass heredity and breeding.In the first part of this thesis the transformation acceptor system for Agrostis stolonifera L., P. pratensis L., Festuca arundinacea Schreb and Lolium perrene L. establishment was performed. In the second part of thesis the factors influencing the particle bombardmental transformation efficiency of three Lolium perrene L. and Agrobacterium-mediated transformation efficiency of three Agrostis stolonifera L. varieties were investigated, and the Bar-Gus gene, NPT â…¡ resistance and nicotianamine synthase genes were transformed into those varieties. In the last part of thesis the drought-resistant identification in transgenic plant of Agrostis stolonifera L. was stated, and the mechanism of NAS gene was discussed.1. lishment of transformation acceptor system for Agrostis stolonifera L., P. pratensis L., Festuca arundinacea Schreb and Lolium perrne L.Several factors affecting turfgrass callus induction, subculture, bud differentiation and plant regeneration were investigated with mature embryos(excising endosperm or treated by 6N HCl) as explants. The results indicated that high ratio of [NO3+-N]/[NH4+-N], numerous inorganic micro-elements with larger amount of L-pro , CH and mannital were the optimal medium elements. Application of different medium plays an important role on the maintenance of embryogenic state of long-term cultured calli. With the optimal 6-BA concentration, supplementing the regeneration medium with NAA was found to apparently improve the frequency and quality of plant differentiation.
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