Development Of Molecular Markers And Population Genetic Analyses In Several Important Fishes Distributed In The Yangtze River System

The Yangtze River is the longest river in China and also the third longest one in the world. With fish species (subspecies) over 360, including about 180 endemic species (subspecies), the Yangtze River system is the region with the highest diversity of fish species in China. Because of over-fishing, water pollution and construction of hydroelectric dams etc., the fishery resources of Yangtze River had declined severely in last twenty years. To better protect and effectively utilize fishery resources in the Yangtze River, molecular genetic analyses in some fishes with economic or ecological importance are necessary, which are also basic to studies on fish ecology, fishery resources and genetic breeding.This dissertation consists of two sections. One is the screening and development of molecular markers of some important Yangtze River fishes as following: 1) Some polymorphic microsatellites from enriched-genomic libraries of Coreius heterodon, Coreius guichenoti, Gobiocypris rarus were isolated and characterized using the Fast Isolation by AFLP of Sequences COntaining repeats (FIASCO) protocol, and cross-species amplifications of these new microsatellites were executed in related species. 2) We attempted to develop Expressed Sequence Tags (EST)- containing microsatellites (EST-SSR) in common carp (Cyprinus carpio) by data mining, and some Type I markers were obtained. 3) RAPD-SCAR markers for species identification of seven species (C. heterodon, C. guichenoti, Pseudorasbora parva,...