Genetic Analyses Of The Spawning Stock Of Chinese Sturgeon (Acipenser Sinensis) And Contribution Assessment Of Artificial Propagation On Natural Juvenile Population Based On Microsatellite Markers

The anadromous Chinese sturgeon (Acipenser sinensis) mainly lives in the continental shelf of the Yellow Sea and East China Sea, and spawns in the Yangtze River and Pearl River. Chinese sturgeon is included in the IUCN Red List of Threatened Species in 1996, and is also under the category I in state protection species list of China. To ensure their future survival, researches using microsatellite markers were done to provide the genetic data on address several specific conservation issues on this endangered species. We aimed to: 1) analyze the inheritance mode of microsatellite loci in polyploidy Chinese sturgeon, 2) evaluate the genetic health level of extant spawning stocks, 3) establish the model which could estimate identifying ability of tetraploid microsatellite loci, and 4) assess the contribution of artificial propagated juveniles to the wild population.1 The inheritance mode of microsatellite loci was tested using three families created from wild Chinese sturgeon. The results showed that five microsatellite loci conformed to co-dominant Mendelian inheritance fashion. Primers As-073, As-100 and Spl-168 expressed a maximum of four even-intensity bands per individual and pairwise combination of alleles segregation in the progeny that fit tetrasomic inheritance (P>0.05). Double reduction exhibited at As-073 simultaneously gave evidence of tetrasomic inheritance. The mode of inheritance at As-074 and Afu-54, both expressing more than two alleles in one individual, could not be ultimately...