The Virulence Genes And Genetic Engineering Vaccines Of Aeromonas Hydrophila

There are six chapters in the present dissertation. As a whole in this paper, thecorrelation between the distribution pattern of virulence genes and the virulence ofAeromonas hydrophila was studied, the full-length aerolysin gene aerA of A.hydrophila XS91-4-1 was cloned ans sequenced, and the recombinant aerolysin wasexpressed in E. coli in an active form. Then the recombinant protein was used toseparate the trypanosomes in the blood successfully. On the other hand, we analyzedthe distribution of the epitopes of the aerolysin and constructed the subunit candidatevaccine and DNA candidate vaccine on the aerolysin of A. hydrophila.In chapter two, the correlation between the distribution pattern of virulencegenes and the virulence of A. hydrophila was analyzed. The presence of threevirulence genes in nine strains was investigated using polymerase chain reaction(PCR) including the genes encoding aerolysin, hemolysin and extracellular serineprotease (i.e. aerA, hlyA and ahpA, respectively). And the pathogenicities of thesestrains were also assayed to Carrassius auratus ibebio. The results showed that there...