Cloning Of Stilbene Synthase Gene And Its Genetic Transformation In Melon(Cucumis Melo L.)

Resveratrol belongs to stilbene in structure, and is one of the most important activity components in stilbene. In early day people did not easily found the trees rotted while containing stilbene, hence resveratrol was called as a"plant fungicide". The trial of modern medical mechanism showed that resveratrol had extensively physiological activity and multiple functions in health care. Research of stilbene is the subject in the plant disease tolerance and the development of plant health care medicine which people pay more attention to. The synthesis of resveratrol and other stilbene compounds are sub-metabolism, via phenylalanine pathway. The stilbene synthase(STS) is the key regulation enzyme.Resveratrol are just in the grapes and peanuts only. Resveratrol synthesis can not be performed without stilbene synthase gene. More and more people pay attention to the transgenic plants of stilbene synthase to improve plant disease tolerance and quality.Polygonum cuspidatum, the highest resveratrol content plant discovered in the nature at present, is the perennial herbaceous plant of polygonaceae. Bauhinia variegata, resveratrol in its fruit legumes, is the greening trees of Leguminosae.Melon is annual herbaceous rambler of Cucurbitaceae, which has aboundantly nutritious fruit, special flavour, and suitable fragrance and sweet hence liked by people and listed in the one of the biggest yield fruits in the world. China, half total area and yield of melon in the world, is the biggest country in producing melon, melon includes two series of the thin-skinned and thick-skinned at present.From the first transgenic plant appeared in 1983, the transgenic technology developed rapidly in exploiting new pathway of producing excellence species of high yield, high quality, pest and disease tolerance, and adversity tolerance. Great success has been done in the transgenic plants, as well as bulky benefit in society and economy. The research in transgenic melons is backwards since the technology is late performed in melons, the tissue culture is difficult in regeneration, and optimization in genetic transformation systems.This paper describes the results and discussion as follows:1. Determine resveratrol content in the several kinds of tree fruits and pharmic plants.By determining over 10 kinds of tree fruits, the results showed resveratrol in the fruits of Morus alba, Ananas cornosus, Syzygium jambos, Vitis vinifera and Archontophoenix alexandrae. The order of content is Morus alba > Ananas cornosus > Syzygium jambos > Vitis vinifera > Archontophoenix alexandrae. From...