Variation Of Chromosomal Structure, Genome And Phenotype Of Wheat Caused By Repetitive DNA Sequence

A major component of the eukaryote nuclear genome is constituted by different classes of repetitive DNA sequences. More and more researches indicated that repetitive DNA sequences played an important role on genomic function. Many researchers thought that repetitive DNA might have an overall impact on the regulation of gene expression and development of organisms. Some researchers studied the effect of repetitive DNA on the discordance of human monozygotic twins and plant siblings.Repetitive DNA sequences of Triticeae crops were also studied and the topics were mainly on the evolution. In this research, by the methods of PCR, clone, sequence, C-banding, GISH, FISH, Southern blot, A-PAGE and SDS-PAGE, we used repetitive DNA sequences at the fist time to investigate the variation of the structure of 1RS.1BL chromosomes of five sets of sister wheat lines. These sister wheat lines derived from a same parental wheat line respectively. We combined the variation of the structure of 1RS.1BL chromosomes with the phenotypic differences between the sister wheat lines derived from the same parental wheat line. We enucleated that repetitive DNA had impacts on phenotype by the way of changing the whole organization of genome. Furthermore, we transformed the rye-specific repetitive DNA sequences into wheat which does not contain the rye-specific repetitive DNA sequences at the first time. Some transformants were obtained and they were also used to enucleate that the variation of genome and phenotype was caused by repetitive DNA sequences. So, a few new results as following were obtained:1.According to the published rye-specific repetitive DNA sequences, pSc119.1 (dispersed), pSc20H (dispersed), pAWRC.1 (centromere) and pAw161 (subtelomere), six pairs of primers, Pr119.1, Pr20H, PrCEN-1 (from 24bp to 1.12kb of pAWRC.1), PrCEN-2 (from 1.14kb to 2.09kb of pAWRC.1) PrCEN-3 (from 2.085kb to 3.265kb of pAWRC.1) and Pr161, were designed. These primers designed, one microsatellite primer SCM9 located on chromosome arm IRS and eight microsatellite primers...