Comparetion Of The Genetic Structure Between Wild And Cultured Populations Of Grass Carp

Grass carp, Ctenopharyngodon idella, which belongs to Cypriniformes, Cyprinidae, Leuciscinae, is a widely cultured freshwater fish because of its herbivorous, fast growth and delicious flesh . The success of its artificial propagation improved the status of its fry in short supply and accelerated the foundation of many small breeding populations. In limited of conditions and scales, many seed farm kept few parents and apart of them were offspring of their inbreeding. Its germ plasm, however, such as prematurity, the decline of hatching ratio, and the ability of disease resistance and spawning has been seriously degenerating every year in recent years.On population genetic study, it became a hot research subject that using different DNA marker to forecast genetic differentiation among different populations. However, we haven't seen such report in fishery research. Because many degenerating character related to loss of rare allele and decrease of heterozygosis, it is a useful and feasible method to use lots of DNA marker to predict decrease of heterozygosis and disbennifit mutation cased by inbreeding among different grasss carp populations.In this paper, we constructed grass carp microsatellite-enriched library and then compared the allele frequency variation among wild and cultured stock of grass carp by using microsatellite or simple sequence length polymorphism, TRAP (target region amplified polymorphism) , SRAP (sequence-related amplified polymorphism) from different part of its genomic and made known the change of inbreeding population's germ plasm. This work supplied the theoretic basis to solve the problem of degenerating and offered many useful genetic markers for the construction of its genetic linkage map in the future.1 Extraction of grass carp genomic DNA and construction the grass carp genomic microsatellite-enriched library.Grass carp genomic DNA was extracted from fin and blood by using traditional method and reagent-box. The result show: the quality of genomic DNA extracted from...