Molecular Marker Development, Genetic Linkage Map Construction And QTL Analysis In Pacific White Shrimp (Litopenaeus Vannamei)

A genetic linkage map was constructed using amplified fragment length polymorphism (AFLP) and microsatellite markers. One hundred and eight selected AFLP primer combinations and 30 polymorphic microsatellite markers produced 2071 markers that were polymorphic in either of the parents and segregated in the progeny. Putative SNPs were found by sequence alignment such as BGBP, LGBP and MIH gene fragments between parents of the mapping family.Of these segregating markers, 319(including 18 microsatellite loci) were mapped to the female framework map that contained 45 linkage groups that ranged in size from 28.5 cM to 260.0 cM and consisted of between 4 and 16 markers (Figure 1, Table 3). The total linkage distance spanned by these markers was 4134.4 cM and the average spacing of each group was 7.6-25.9 cM with an average of 15.1 cM for the whole framework map; and 267 were assigned to 45 linkage groups, with 252 AFLP loci and 14 microsatellite loci placed at precise positions covering 3220.9cM. The length of each group varied from 14.2 to 161.1 cM Kosambi. The average spacing of...