| Helicoverpa armigera belongs to Noctuidae, Lepidoptera, which seriously influences crop production in our country because of its great appetite, multiform food and high proliferation. Chemical medication makes pests drug-fast, pollutes the environment and brings harm to people simultaneously. By studies in vivo and in vitro, we can understand the mechanisms of insect development. Then we can interfere with their development and reproduction or use the pathogens to kill pests according to these biological mechanisms. Biocontrol is a very good strategy because of their high specificity on pests and harmlessness for human, livestocks and the environment.In this dissertation, two insecticides of recombinant baculovirus with two kinds of Helicoverpa armigera functional genes were studied respectively. This study gave evidences to the two new candidate genes of recombinant baculovirus. Three kinds of cell lines of H.armigera were also established which laid foundation for in vitro studying on the mechanisms of gene expression by hormone, infection by virus and apoptosis in live cells. Summarized as followed:First part: the study on insecticides of recombinant baculovirus of Helicoverpa armigera functional genesBaculoviruses are considered as ideal alternative agents for pest control, which are insects specific pathogen and cannot infect vertebrate. But wild type baculoviruses have a narrow spectrum hosts and slowly killing speed according to the studies of their infecting processes in cells or in insects. To improve their insecticidal activity, baculoviruses are often modified by gene manipulation on purpose. Although recombinant baculoviruses express insect-selective toxin genes, development-disrupting enzyme gene or hormone gene showed that they could kill insects faster than wild-type baculovirus and reduce feeding damages. It is necessary to find powerful and environmentally safe genes to genetically...
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