| Poplar is an important fast growing timber tree species which has significanteconomic and ecological benefits. However it is threatened by pathogens or insectpests during growing periods. Black spot disease in poplar is a leaf disease causedby the fungus. The chief pathogen is Marssonina brunnea f. sp. multigermtubi. Sofar, very little is known about the molecular mechanism of poplar-M. brunneainteraction. In order to identify proteins related to disease resistant and primarilyunderstand molecular bases, the clone "NL895" (P.×euramericana CL"NL895")which is high resistant to M. brunnea f. sp. multigermtubi was used as theexperimental material. We used 2-DE and MS to identify proteins in poplar leavesthat were differentially expressed in response to black spot disease pathogen,Marssonina brunnea f. sp. multigermtubi. Proteins. were extracted from poplarleaves at 0, 12, 24, 48, 72 h after pathogen inoculation and separated by 2-DE.The gels were analyzed by PDQuestTM software (Bio-Rad). About five hundredreproducible protein spots were detected, among which forty protein spotsdisplayed differential expression and were subjected to MALDI-TOF MSfollowed by database searching. The identified proteins mostly include putativeascorbate peroxidase, S-adenosyl methionine synthetase, calmodulin bindingprotein, quinone oxidoreductase-like protein, Lysine-ketoglutaratereductase/saccharopine dehydrogenase, F-box protein, UMP/CMP kinase,ethylene response sensor, translation initiation factor. These proteins may beclosely related to plant defense response to pathogen. In addition, some of theidentified proteins belong to RuBisCO family. Most of these proteins showeddrastic reduction under pathogen stress. According to the function, the identifiedproteins were sorted into five categories, including photosynthesis, proteinsynthesis, metabolism, defense response and unclassified proteins. Finally,biological functions of the proteins were discussed. |
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