Antifungal Activity Of Thymol And The Global Gene Expression Profile Of The Model Fungus Saccharomyces Cerevisiae Induced By Thymol

Clinical infectious diseases by bacteria, fungi or viruses cause three main types of micro-organisms, so the treatment of fungal infections is an important part of clinical anti-infective treatment. In recent years, with tumor and AIDS patients increasing, organ transplants, and duct intubation and endoscope technology to carry out widespread and a wide range of applications of efficient broad-spectrum antibiotics, immunosuppressants and glucocorticoids, more and more often fungal infections see. Clinically, many years amphotericin B, and azole drug are special effect and first-choice drugs of treatment of fungal infections. However, renal toxicity caused by amphotericin B, narrow-spectrum treatment of azole drugs and emergence of drug-resistant strains, making an in-depth study of fungus-resistant mechanisms, and to overcome and slow down the development of drug resistance, the development of new safe and effective antifungal agents are particularly prominent. China's rich traditional Chinese medicine resources in the search for effective antifungal agents has aroused widespread attention. Thymol (Thymol), from the plants of thyme and oregano and tangerine peel. Research shows that thymol widely used anti-bacterial, anti-corrosion and disinfection. It is worth noting that it has significant activity of disease-resistant pathogenic fungi.Global gene expression profile can be analyzed quickly and systematically in the whole genome level of gene expression by use of DNA microarray. And through monitoring the concentration of mRNA can be done specifically for each gene analysis. Using microarrays on a biological analysis of all the genes in parallel model for the understanding of gene function can provide some clues (target identification). Yeast as a model eukaryotic, has many similarities with higher eukaryotic life in the adjustment mechanism and is a ideal model to study the mechanism of antifungal agents.This study researched on in vitro drug sensitivity of thymol against clinical isolates of fungi alone and in combination with fluconazole and amphotericin B. Notablely, this study in the international arenas for the first time analyzed the expression profile of thymol effected on Saccharomyces cerevisiae and explain the molecular mechanism of thymol effected on Saccharomyces cerevisiae. Furthermore, the results of DNA microarray has been verified by using RT-PCR and chip-related phenotype tcDNA has been done. The results show that thymol fluconazole and significantly synergy when thymol was in combination with fluconazole and amphotericin B; The results of gene expression profile show that the 517 genes differentially expressed, 305 genes upregulated and 212 genes downregulated. On this basis, we used advanced cluster analysis software, T-profiler to analyze the data. At the same time, RT-PCR tcDNA showed the good results consistent with the results of the microarray. In addition, glucose-dependent efflux of rhodamine 6G from Saccharomyces cerevisiae showed that thymol can activate efflux pump of Saccharomyces cerevisiae.