Study On Immunological Stress During Vaccination And Preparetion And Application Of Anti-stress Agent In Domestic Animals And Fowls

To cope with immunological stress during vaccination in production of animals and fowls in pig and chicken were used as experimental models, mechanism and regulation for immunological stress of swine fever (SF) , foot and mouth disease, porcine reproductive and respiratory syndrome (PRRS) , Newcastle disease (ND) vaccination were studied, and their anti-stress agents were prepared respectively. The results were as follows.1) Vaccination by using Foot and mouth disease vaccine, porcine reproductive and respiratory syndrome vaccine, swine fever vaccine and Newcastle disease vaccine II could cause immunological stress in animals and fowls production.2) Based on the study of chicken model, it was shown that one of mechanisms for immunological stress induced by vaccination was regulatory effect of Corticotropin-releasing hormone (CRH) and Thyrotropin-releasing hormone (TRH) gene expression. The study confirmed that CRH mRNA and TRH mRNA in hypothalamus were significantly expressed, and regulated secretion of adrenocorticotropic hormone (ACTH) and cortisol, which finally led to immunological stress.3) Astaxanthin had effect of anti-stress and immunological regulation during vaccination of Newcastle disease vaccine II, it could cause the decrease of secretion of CRH and TRH by regulating gene expression of CRH mRNA and TRH mRNA, which finally led to the decrease of immunological stress response. Moreover, it could cause the increase of daily gain for broilers.4) Glutamine played an important role in anti-stress and immunological regulation during vaccination of Newcastle disease vaccine II, it could cause the significant decrease of secretion of CRH and TRH by regulating expression of CRH mRNA and TRH mRNA in hypothalamus, moreover, daily gain of broilers, antibody titer and concentration of lysozyme in sera were significantly increased, organ index of spleen and thymic were very significantly increased.5) Anti-immunological stress agents "Cumian No.1" was prepared, it could alleviate immunological stress of pig and chicken, increase daily gain, survival rate, feed conversion ratio (FCR) and antibody titer of pig and chicken. Anti-immunological stress agents ASFP-1 for foot and mouth disease vaccination was prepared, it could prevent allergy and fervescence, significantly increase daily gain and decrease feed conversion ratio of pig. It could significantly decrease concentration of ACTH and cortisol in sera, meanwhile not affect antibody titer in sera. Immunoenhancement regulator of PRRS vaccination was also prepared, it could alleviate symptoms of immunological stress caused by PRRS vaccination, IgG concentration in sera was significantly increased by injecting Immunoenhancement regulator agents IRFF of PRRS, formation of neutralizing antibody is 10 days aheadThe research confirmed that inoculation vaccine for ainmals and fowls were special stress sources, one of it's mechanisms could be significant regulatory effect of CRH and TRH mRNA expression in hypothalamus. The results showed Astaxanthin and Glutamine played important roles in anti-immunological stress and immune regulation. The products for Anti-immunological stress agents "Cumian No.1", anti-immunological stress agents ASFP-1 of foot and mouth disease vaccination and immunoenhancement regulator of PRRS vaccination were successfully prepared, 2 invention patents have been successfully obtained...