The Synthesis Regularity Of Active Components And Pharmaceutical Research Of Osmanthus Fragrans Lour.

The flower of Osmanthus Fragrans Lour. is of economic importance because it isused as expensive perfumes and traditional medicine. Presently, studies on applicationdevelopment and pharmacy of O. fragrans were rarely reported. In this paper, we studiedthe active components' synthesis conversion and pharmaceutical of O. fragrans. It couldprovide information for people to select the reasonable harvest stages, storage methodsand application of O. fragrans resource. The results of the research were as follows:(1) GC fingerprint of essential oil of O. fragrans was established using computersoftware. It was effective to evaluate the quality of O. fragrans. Latifolius Group was thebest germplasm resources for perfumery production. We compared contents of aminoacids, trace elements of different varieties of O. fragrans. The result showed thatThunbergii group had better nutritional value and Aurantiaeus group had better ecologicaladaptability.(2) We studied the rules of synthesis and conversion of essential oil. The resultshowed that the initial flowering stage was the best period for picking. The yield ofessential oil was higher and the main aroma components were also higher at the initialflowering stage. In this paper, we studied the physiological metabolism of O. fragrans andexplained the formation of the fragrance by using optical electron microscopy, scanningelectron microscopy and transmission electron microscopy.(3) We investigated the changes of the yield and chemical compositions of O.fragrans stored at different conditions. Adding citric acid could significantly increase theyield of essential oil and the best add volume was 0.1%-0.5 %. The result could givesome practical guidance for the utilization of O. fragrans.(4) The technology of extraction and purification of active components of O.fragrans were studied. Solvent extraction was the best method for essential oil. Theextracting parameters of O. fragrans concrete were determined using Response SurfaceMethododlogy. The best extraction conditions were the temperature of 24.5℃, extractiontime 110 min, the solid-liquid ratio of 1:2.7. The extraction techniques for total flavonoidsof O. fragrans were investigated by contrast. The results showed that release andhot-extraction method was better than other extractions. The optimum purification conditions of total flavonoids with macroporous resin HPD400 were obtained. Withmacroporous resin to adsorb and purify, the yield of total flavonoids was higher than 90 %and the content was higher than 80 %.(5) The biological activity and pharmacological effects of concrete and totalflavonoids of O. fragrans were studied. We found that total flavonoids of O. fragrans hhigh anti-bacterial and anti-oxidation effects. The lipid antioxidant experiments showedthat total flavonoids of O. fragrans had better anti-oxidation effects in lard, rapeseed oil,soya bean oil, peanut oil. Synergist, such as ascitric acid and Vc, could enhance itsantioxidant effect. With antioxidants, such as BHA, BHT and PG, could enhance itsantioxidant effects.Effects of concrete and total fiavonoids of O. fragrans on liver injury induced bycarbon tetrachloride in rats were studied. The concrete of O. fragrans had excellent effectof the treatment of liver damage. Liver biopsy revealed that O. fragrans concrete of large(600 mg/kg), medium (300 mg/kg), low-dose (150 mg/kg) groups had excellenttherapeutic effect for balloon cell degeneration and necrosis. It was better than thebiphenyldimethylesterate (200 mg/kg). O. fragrans has the the potential of healthy foodsand drugs.